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Ligand-mediated transcription elongation control using triplex-based padlock oligonucleotides.
Chem Biol
April 2004
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U565, CNRS UMR5153, 43 rue Cuvier, 75231 Paris Cedex 05, France.
Triplex-forming oligonucleotides (TFOs) provide useful tools for the artificial regulation of gene expression at the transcriptional level. They can become topologically linked to their DNA target upon circularization, thereby forming very stable triple helical structures. These "padlock oligonucleotides" are able to interfere with transcription elongation when their target site is located in the transcribed region of a gene.
View Article and Find Full Text PDFA Ligand-Modulated Padlock Oligonucleotide for Supercoiled Plasmids T.R. was the recipient of a Bourse de Docteur Ingénieur from the CNRS. We are grateful to Prof. T. Garestier and J. S. Sun for helpful discussions, and to Dr. C. H. Nguyen for a gift of BQQ.
Angew Chem Int Ed Engl
April 2001
Laboratoire de Biophysique, INSERM U201 CNRS UMR8646 43, rue Cuvier, 75231 Paris Cedex 05 (France).
A Ligand-Modulated Padlock Oligonucleotide for Supercoiled Plasmids.
Angew Chem Int Ed Engl
April 2001
Laboratoire de Biophysique, INSERM U201 CNRS UMR8646 43, rue Cuvier, 75231 Paris Cedex 05 (France) Fax: (+33) 1-40-79-37-05.
Catenation of a circular oligonucleotide to a supercoiled plasmid can be achieved in high yields by means of ligand-induced triple-helix formation. The noncovalent interactions in this supramolecular structure can be modulated by a triplex-stabilizing agent (TSA; see picture). The circular oligonucleotide represents a noncovalent anchor for plasmid functionalization.
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