Sarcocysts were dissected from the tongue of a nine-banded armadillo (Dasypus novemcinctus). DNA was extracted and characterised by PCR amplification followed by restriction fragment length polymorphism analysis and nucleotide sequencing. A total of 1879 nucleotides were compared; the sarcocyst DNA sequence was identical to that reported for Sarcocystis neurona. DNA was extracted from the sarcocysts of five more nine-banded armadillos. A 254-nucleotide sequence was determined for each and found to be identical to S. neurona. Western blot techniques for detection of anti-S. neurona antibody were developed for use with armadillo plasma and samples from 19 wild-caught and 17 captive-raised armadillos were examined. Whereas all of the 19 wild-caught armadillos had antibodies to S. neurona, only one of 17 captive-raised armadillos did. These results suggest that the nine-banded armadillo are naturally infected with S. neurona.
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http://dx.doi.org/10.1016/s0020-7519(01)00178-3 | DOI Listing |
Acta Trop
December 2024
Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction, and One Health, School of Agricultural and Veterinary Sciences, São Paulo State University "Júlio de Mesquita Filho" (FCAV/UNESP), Jaboticabal Campus, Via de Acesso Prof. Paulo Donato Castellane, s/n, Rural Zone, Jaboticabal, SP, CEP: 14884-900, Brazil. Electronic address:
We found Mycobacterium leprae, the most common etiologic agent of Hansen disease or leprosy, in tissues from 9 (18.75%) of 48 nine-banded armadillos (Dasypus novemcinctus) collected across continental Ecuador. Finding evidence of a wildlife reservoir is the first step to recognizing leprosy zoonotic transmission pathway in Ecuador or elsewhere.
View Article and Find Full Text PDFBraz J Infect Dis
October 2024
Universidade Federal de Mato Grosso, Faculdade de Medicina Veterinária, Departamento de Microbiologia e Biologia Molecular, Cuiabá, MT, Brazil.
Leprosy is a chronic infectious disease caused by the bacilli Mycobacterium leprae and Mycobacterium lepromatosis. In addition to humans, animals such as nine-banded armadillos and red squirrels are species naturally infected. The objective of this study was to investigate the presence of M.
View Article and Find Full Text PDFHum Reprod Update
December 2024
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.
Background: Monozygotic (MZ) twins are believed to arise from the fission of a single fertilized embryo at different stages. Monochorionic MZ twins, who share one chorion, originate from the splitting of the inner cell mass (ICM) within a single blastocyst. In the classic model for dichorionic MZ twins, the embryo splits before compaction, developing into two blastocysts.
View Article and Find Full Text PDFSyst Biol
June 2024
Institut des Sciences de l'Evolution de Montpellier (ISEM), Univ. Montpellier, CNRS, IRD, Montpellier, France.
The nine-banded armadillo (Dasypus novemcinctus) is the most widespread xenarthran species across the Americas. Recent studies have suggested it is composed of four morphologically and genetically distinct lineages of uncertain taxonomic status. To address this issue, we used a museomic approach to sequence 80 complete mitogenomes and capture 997 nuclear loci for 71 Dasypus individuals sampled across the entire distribution.
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