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A technology platform for standardized cryoprotection and freezing of large-volume brain tissues for high-resolution histology.
Front Neuroanat
November 2023
Department of Electrical Engineering, Indian Institute of Technology Madras, Chennai, India.
Understanding and mapping the human connectome is a long-standing endeavor of neuroscience, yet the significant challenges associated with the large size of the human brain during cryosectioning remain unsolved. While smaller brains, such as rodents and marmosets, have been the focus of previous connectomics projects, the processing of the larger human brain requires significant technological advancements. This study addresses the problem of freezing large brains in aligned neuroanatomical coordinates with minimal tissue damage, facilitating large-scale distortion-free cryosectioning.
View Article and Find Full Text PDFAdvancements in 3D spheroid imaging: Optimised cryosectioning and immunostaining techniques.
MethodsX
December 2023
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M DK-5230, Denmark.
This article presents a modified protocol for embedding and sectioning spheroids and organoids, which are increasingly used in research due to their ability to emulate living tissue. The modifications aim to reduce the distortion and damage of these fragile structures during the embedding and sectioning process. The new method involves using optimized embedding containers, a modified embedding protocol, and optimized temperatures for cryosectioning.
View Article and Find Full Text PDFQualitative Comparison of Cryostat- versus Snap-Frozen Neurosurgical Intraoperative Consultations.
Int J Surg Pathol
September 2023
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Frozen sections (FS) are common in neurosurgery to address varied clinical concerns. Artifacts in central nervous system (CNS) FS can be severe and affect or hinder interpretation. We performed a case-control study using a semiquantitative scale: the Histologic Preservation Score (HPS), and a quantitative scale: the Ice Crystal Vacuolization Score (ICVS), to compare the histologic quality yielded by snap- versus cryostat freezing techniques.
View Article and Find Full Text PDFEx vivo fluorescence confocal microscopy: chances and changes in the analysis of breast tissue.
Diagn Pathol
June 2022
Department of Pathology, Medical University of Vienna, Vienna, Austria.
Background: Rapid histologic diagnosis of frozen sections is essential for a variety of surgical procedures. Frozen sections however, require specialized lab equipment, are prone to freezing artifacts and are not applicable to all types of tissue. Adipose tissue is especially difficult to process in frozen sections.
View Article and Find Full Text PDFDIUTHAME enables matrix-free mass spectrometry imaging of frozen tissue sections.
Rapid Commun Mass Spectrom
May 2020
The Graduate School for the Creation of New Photonics Industries, 1955-1 Kurematsu-cho, Nishi-ku, Hamamatsu, Japan.
Rationale: A recently developed matrix-free laser desorption/ionization method, DIUTHAME (desorption ionization using through-hole alumina membrane), was examined for the feasibility of mass spectrometry imaging (MSI) applied to frozen tissue sections. The permeation behavior of DIUTHAME is potentially useful for MSI as positional information may not be distorted during the extraction of analytes from a sample.
Methods: The through-hole porous alumina membranes used in the DIUTHAME chips were fabricated by wet anodization, were 5 μm thick, and had the desired values of 200 nm through-hole diameter and 50% open aperture ratio.
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