Neisseria cuniculi produces the restriction enzyme NcuI which is an isoschizomer of MboII. We have demonstrated that NcuI recognizes a pentanucleotide sequence (5'-GAAGA-3'/3'-CTTCT-5'), and cleaves the DNA 8 and 7 nucleotides downstream from the recognition site leaving a single 3'-protruding nucleotide. We have purified this enzyme to electrophoretic homogeneity using a four-step chromatographic procedure. NcuI endonuclease is a monomeric protein with a M(r)=48,000+/-1000 under denaturing conditions. The properties of NcuI are consistent with those for MboII, the position of the cleavage site being identical and the pH profile and divalent cation requirements being similar. Moreover, NcuI cross-reacts strongly with anti-MboII serum suggesting the presence of similar antigenic determinants. We have determined the sequence of 20 N-terminal amino acids for NcuI and concluded that this sequence is identical to the N-terminal portion of the MboII enzyme.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1574-6968.2001.tb10560.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
M1.MboII and M2.MboII type IIS methyltransferases: different specificities, the same target.
Microbiology (Reading)
April 2009
Department of Microbiology, University of Gdansk, 80-822 Gdansk, Kladki 24, Poland.
Methylation of a base in a specific DNA sequence protects the DNA from nucleolytic cleavage by restriction enzymes recognizing the same sequence. The MboII restriction-modification (R-M) system of Moraxella bovis ATCC 10900 consists of a restriction endonuclease gene and two methyltransferase genes. The enzymes encoded by this system recognize an asymmetrical sequence 5'-GAAGA-3'/3'-CTTCT-5'.
View Article and Find Full Text PDFMolecular characterization of the DNA methyltransferase M1.NcuI from Neisseria cuniculi ATCC 14688.
Res Microbiol
March 2007
Department of Microbiology, University of Gdansk, 80-822 Gdansk, Kladki 24, Poland.
The methyltransferase M1.NcuI is a member of the restriction-modification system in Neisseria cuniculi ATCC14688 and recognizes the asymmetric pentanucleotide sequence 5'-GAAGA-3'/3'-CTTCT-5'. We purified M1.
View Article and Find Full Text PDFInfectious bovine keratoconjunctivitis and lymphofollicular hyperplasia of the third eyelid in heifers.
J Vet Med B Infect Dis Vet Public Health
March 2001
Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot 76100, Israel.
On a dairy cattle farm, infectious bovine keratoconjunctivitis was diagnosed in 29 (24%) calves and heifers aged from 2 weeks to 1 year old. The highest infection rate (18%) occurred in animals aged 3-6 months. The bacteriological examination of swabs from the affected animals yielded several species of bacteria: Moraxella bovis, Neisseria ovis, N.
View Article and Find Full Text PDFIsolation and characterization of type IIS restriction endonuclease from Neisseria cuniculi ATCC 14688.
FEMS Microbiol Lett
March 2001
Department of Microbiology, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland.
Neisseria cuniculi produces the restriction enzyme NcuI which is an isoschizomer of MboII. We have demonstrated that NcuI recognizes a pentanucleotide sequence (5'-GAAGA-3'/3'-CTTCT-5'), and cleaves the DNA 8 and 7 nucleotides downstream from the recognition site leaving a single 3'-protruding nucleotide. We have purified this enzyme to electrophoretic homogeneity using a four-step chromatographic procedure.
View Article and Find Full Text PDFA numerical phenotypic taxonomic study of the genus Neisseria.
Microbiology (Reading)
October 1994
Department of Microbiology and Immunology, Leicester University, UK.
A numerical phenotypic taxonomic study of 315 strains of Neisseria and some allied bacteria examined for 155 phenotypic tests showed 31 groups, most of which were reasonably distinct. These fell into four major areas. Areas A, B and C contained species of Neisseria, whereas area D contained the organisms known as 'false neisserias' together with Branhamella, Moraxella and Kingella species.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!