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and CD8 T Cell Suppression Assays.
Bio Protoc
May 2021
Department of Urology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Guangzhou, China.
CD8CD28 T suppressor cells (Ts) have been documented to promote immune tolerance by suppressing effector T cell responses to alloantigens following transplantation. The suppressive function of T cells has been defined as the inhibitory effect of Ts on the proliferation rate of effector T cells. H-thymidine is a classical immunological technique for assaying T cell proliferation but this approach has drawbacks such as the inconvenience of working with radioactive materials.
View Article and Find Full Text PDFHuman CD8CD28 T suppressor cells expanded by common gamma chain (γc) cytokines retain steady allospecific suppressive capacity in vivo.
BMC Immunol
April 2020
Department of Immunology, School of Basic Medical Science, Southern Medical University, No.1023-1063 Shatainan Road, Guangzhou, 510515, Guangdong, China.
Background: CD8CD28 T suppressor (Ts) cells play critical role in transplant tolerance. Our previous study has generated CD8CD28 Ts cells in vitro which exert robust allospecific suppressive capacity in vitro.
Results: CD8CD28 Ts cells were expanded by stimulating human CD8 T cells with allogeneic antigen presenting cells in the presence of the common gamma chain cytokines IL-2, IL-7 and IL-15 in vitro, and were further verified in vitro through day 7 to 11 for their persistency of the allospecific suppressive capacity.
Human CD8CD28 T Suppressor Cells Expanded by IL-15 Suppress in an Allospecific and Programmed Cell Death Protein 1-Dependent Manner.
Front Immunol
June 2018
Department of Urology, Guangdong General Hospital (Guangdong Academy of Medical Sciences), Guangzhou, China.
CD8CD28 T suppressor cells (Ts) have been recently documented to play an important role in alloimmunity. Therefore, understanding and optimizing the conditions under which these cells are generated and/or expanded would greatly facilitate further research and potential clinical use. In this study, we describe rapid expansion of human allospecific CD8CD28 Ts cells through coculture of CD8 T cells with human leukocyte antigen-mismatched donor antigen-presenting cells plus IL-15 in a relative short period of time .
View Article and Find Full Text PDFAllospecific CD8 T suppressor cells induced by multiple MLC stimulation or priming in the presence of ILT3.Fc have similar gene expression profiles.
Hum Immunol
February 2014
Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, United States. Electronic address:
Alloantigen specific CD8 T suppressor cells can be generated in vitro either by multiple stimulations of CD3 T cells with allogeneic APC or by single stimulation in primary MLC containing recombinant ILT3.Fc protein. The aim of the present study was to determine whether multiple MLC stimulation induced in CD8(+) CD28(-) T suppressor cells molecular changes that are similar to those observed in CD8 T suppressor cells from primary MLC containing ILT3.
View Article and Find Full Text PDFTolerogenic dendritic cells and induction of T suppressor cells in transplant recipients.
Methods Mol Biol
February 2014
Department of Pathology & Cell Biology, Columbia University, New York, NY, USA.
Tolerogenic antigen presenting cells (APC), primarily dendritic cells (DC), are essential to the induction and maintenance of immunologic tolerance in clinical transplantation. They induce the differentiation of CD8+ T suppressor (Ts) and CD4+ T regulatory (Treg) or anergic cells, which prevent transplant rejection maintaining a state of quiescence. Tolerogenic APC express high levels of inhibitory receptors such as Immunoglobulin-like transcript (ILT)3 and 4 which inhibit the effector function of T cells that recognize HLA-peptide complexes on APC.
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