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Embedding a ribonuclease in the spore crust couples gene expression to spore development in Bacillus subtilis.
Nucleic Acids Res
January 2025
EGM CNRS, Université Paris-Cité,Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France.
Faced with nutritional stress, some bacteria form endospores capable of enduring extreme conditions for long periods of time; yet the function of many proteins expressed during sporulation remains a mystery. We identify one such protein, KapD, as a 3'-exoribonuclease expressed under control of the mother cell-specific transcription factors SigE and SigK in Bacillus subtilis. KapD dynamically assembles over the spore surface through a direct interaction with the major crust protein CotY.
View Article and Find Full Text PDFNanogap-Assisted SERS/PCR Biosensor Coupled Machine Learning for the Direct Sensing of in Food.
J Agric Food Chem
January 2025
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
() is the primary risk factor in food safety. Herein, a nanogap-assisted surface-enhanced Raman scattering/polymerase chain reaction (SERS/PCR) biosensor coupled with a machine-learning tool was developed for the direct and specific sensing of S. aureus in milk.
View Article and Find Full Text PDFMARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA.
Nat Commun
January 2025
Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Article Synopsis
- The study focuses on the role of a newly discovered protein family called MARTRE in regulating the poly(A) tail length of maternal mRNA during early embryo development in mice.
- MARTRE proteins inhibit the deadenylase CCR4-NOT, helping to maintain longer poly(A) tails and enhance mRNA translation efficiency.
- Deleting the Martre genes leads to shortened poly(A) tails, reduced mRNA translation, and delays in early embryonic development, emphasizing the importance of MARTRE in the translation of maternal mRNA.
Targeting mitochondrial RNAs enhances the efficacy of the DNA-demethylating agents.
Sci Rep
December 2024
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.
Hypomethylating agents (HMAs) such as azacytidine and decitabine are FDA-approved chemotherapy drugs for hematologic malignancy. By inhibiting DNA methyltransferases, HMAs reactivate tumor suppressor genes (TSGs) and endogenous double-stranded RNAs (dsRNAs) that limit tumor growth and trigger apoptosis via viral mimicry. Yet, HMAs show limited effects in many solid tumors despite the strong induction of TSGs and dsRNAs.
View Article and Find Full Text PDFThe effector PieF modulates mRNA stability through association with eukaryotic CCR4-NOT.
mSphere
January 2025
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
Unlabelled: The eukaryotic CCR4-NOT deadenylase complex is a highly conserved regulator of mRNA metabolism that influences the expression of the complete transcriptome, representing a prime target for a generalist bacterial pathogen. We show that a translocated bacterial effector protein, PieF (Lpg1972) of , directly interacts with the CNOT7/8 nuclease module of CCR4-NOT, with a dissociation constant in the low nanomolar range. PieF is a robust inhibitor of the DEDD-type nuclease, CNOT7, acting in a stoichiometric, dose-dependent manner.
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