Methods are described to establish distinct cell cultures from bovine placental cotyledon. The villous tissue of the bovine placental cotyledon is collected and dissociated with 0.125% trypsin. The cells are then cultured in three different media: a serum-free medium, a growth factor supplemented medium, and a medium with 10% serum. A polygonal cell line grew out of the serum-containing medium, a fan-shaped cell line grew out of the serum-free medium, and an epitheloid cell line grew out of the growth factor supplemented medium. These cells maintained their morphology when grown in serum containing medium. The preference of distinct cells for different media in vitro reflects the in vivo physiological regulation of these cells. These distinct cultures re ideal to study the extrinsic and interactive factors in bovine placenta.
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