The aim of the study was to assess the reproducibility of recently developed laser fluorescence method for occlusal caries detection in vivo. 71 non-cavitated premolar and molar teeth were examined repeatedly by three dentists using DIAGNOdent (KaVo) instrument. The presence of caries has been established on the classification of Lussi, created on the basis of "in vitro" investigations. The reproducibility of the measurements were expressed in rank correlation of Spearman. The intra- and interexaminer reliability was also determined by Kappa statistics. The Spearman correlation coefficiencies were fairly high (0.738-0.949) The intraobserver and interobserver coincidencies reflected by Kappa values (0.60-0.88 and 0.44-0.77, respectively) were also convincing.
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Zhonghua Kou Qiang Yi Xue Za Zhi
January 2025
Department of Stomatology, Affiliated Hospital of Putian University, Putian 351106, China.
To investigate the effect of the sizes of osteon-like concentric microgroove structures on the osteoclastic differentiation of macrophages on titanium surfaces, and to provide reference for the surface modification of implants. The silicon wafers sputtered with titanium were selected as the control group (smooth surface specimens) and four concentric groups (concentric circles with the maximum diameter of 200 μ m, the minimum diameter of 20 μ m, the spacing of concentric circles of 10 or 30 μm, the width of microgrooves of 10 or 30 μm, and the depth of microgrooves of 5 or 10 μm) specimens (the total sample size in each group was 27). The width of microgrooves of C10-5 and C10-10 groups was 10 μm, the depth was 5 and 10 μm, and the width of microgrooves of C30-5 and C30-10 groups was 30 μ m, the depth was 5 and 10 μ m, respectively.
View Article and Find Full Text PDFACS Sens
January 2025
Research Institute for Light-induced Acceleration System (RILACS), Osaka Metropolitan University, 1-2 Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan.
The unique characteristics of biological structures depend on the behavior of DNA sequences confined in a microscale cell under environmental fluctuations and dissipation. Here, we report a prominent difference in fluorescence from dye-modified single-stranded DNA in a light-induced assembly of DNA-functionalized heterogeneous probe particles in a microwell of several microliters in volume. Strong optical forces from the Mie scattering of microparticles accelerated hybridization, and the photothermal effect from the localized surface plasmons in gold nanoparticles enhanced specificity to reduce the fluorescence intensity of dye-modified DNA to a few %, even in a one-base mismatched sequence, enabling us to clearly highlight the single nucleotide polymorphisms in DNA.
View Article and Find Full Text PDFAnalyst
January 2025
Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Institute of Functional Nano and Soft Materials (FUNSOM), Soochow University, Suzhou, Jiangsu Province 215123, China.
The choices of matrices and protocols for sample deposition are critical factors, which impact each other in the matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI). Previous reports on MALDI MS matrices have only compared their performances in terms of their MS signal intensities and provided optical microphotos or MALDI MS images of sample spots but typically lacked quantitative evaluation. Therefore, there is an urgent need to develop a multivariate model to evaluate the performance of different combinations of matrices and sample protocols.
View Article and Find Full Text PDFRev Sci Instrum
January 2025
Department Spins in Energy Conversion and Quantum Information Science (ASPIN), Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Hahn-Meitner-Platz 1, 14109 Berlin, Germany.
Confocal microscopy is an invaluable tool for studying fluorescent materials and finds a wide application in biology and in quantum sensing. Usually, these experiments are performed under ambient conditions, but many materials are air sensitive (for example, black phosphorus) and degrade quickly under the strong laser irradiance. Here, we present a glovebox-integrated confocal microscope designed for nitrogen-vacancy (NV) center-based nano-scale sensing and NMR spectroscopy in an inert gas atmosphere.
View Article and Find Full Text PDFFront Nutr
January 2025
College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
Introduction: Frozen pork can reduce the quality of the meat and alter the digestibility and bioavailability of meat proteins in the human body. In this study, we investigated the changes in the basic composition during frozen storage and their effects on the structural properties of digestion products after protein digestion.
Methods: The impacts of frozen storage at different temperatures (-8, -18, -25, and -40°C) and for different times (1, 3, 6, 9, and 12 months) on the basic components and digestive characteristics of pork were evaluated.
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