Background: Mouse imprinted gene Peg3 encodes a large C2H2 type zinc finger protein with unique characteristics. Peg3 knockout mice were found to show an impairment in maternal behaviour of the adult female. Mouse Peg3 is located on the proximal region of chromosome 7 which is syntenic to the long arm of human chromosome 19. It has been reported that a loss of heterozygosity (LOH) of chromosome 19q occurs frequently in several glioma types.
Results: We isolated human PEG3 cDNA. Both human and mouse PEG3 were strongly expressed in the adult brain and the Peg3 protein was localized in the nuclei of both neurones and glial cells. A significant decrease in PEG3 expression was more commonly observed in glioma cell lines as compared with that in primary cultures of astrocytes. Transfection of PEG3 cDNA in a glioma cell line resulted in a loss of tumorigenicity in nude mice.
Conclusions: The human PEG3 gene is a paternally expressed imprinted gene. Introduction of PEG3 cDNA into the glioma cells suggests that human PEG3 protein functions as a tumour suppressor. Human PEG3 is located on 19q13.4 and is one of the candidates for tumour suppressor genes that are predicted to be sited in gliomas.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1046/j.1365-2443.2001.00412.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Impact of excess sugar on the whole genome DNA methylation pattern in human sperm.
Epigenomics
December 2024
Epigenetics and Diabetes Unit, Department of Clinical Sciences in Malmö, Lund University Diabetes Centre, Lund University, Scania University Hospital, Malmö, Sweden.
Aims, Patients & Methods: Dietary factors may regulate the epigenome. We aimed to explore whether a diet intervention, including excess sugar, affects the methylome in human sperm, and to describe the sperm methylome. We used Whole Genome Bisulfite Sequencing (WGBS) to analyze DNA methylation in sperm taken at three time points from 15 males during a diet intervention; i) at baseline, ii) after one week on a standardized diet, and iii) after an additional week on a high-sugar diet providing 150% of their estimated total energy expenditure.
View Article and Find Full Text PDFEnhancing Membrane Permeability of Fluorescein-Type Chromophore Through Covalent Attachment of Chlorinated Dodecaborate.
Molecules
November 2024
Research Initiative for Supra-Materials (RISM), Shinshu University, Ueda 386-8567, Japan.
Anionic boron clusters, such as [BX] (X = Cl, Br, I), have attracted attention in pharmaceuticals due to their unique superchaotropic properties. In particular, [BBr] () has demonstrated strong interactions with biomolecules, facilitating cargo translocation across plasma membranes. In this study, we investigated the effect of covalently attaching chlorinated dodecaborate moiety [BClO-] to 6-carboxyfluorescein (6-FAM) () via a PEG3 linker to form conjugate ().
View Article and Find Full Text PDFMethylation aberrations in partner spermatozoa and impaired expression of imprinted genes in the placentae of early-onset preeclampsia.
Placenta
December 2024
Department of Neuroendocrinology, National Institute for Research in Reproductive and Child Health, Mumbai, 400012, India. Electronic address:
Introduction: Disturbed paternal epigenetic status of imprinted genes has been observed in infertility and recurrent spontaneous abortions. Shallow placentation has been associated with early-onset preeclampsia. Hence, the present study aimed to investigate the methylation patterns of imprinted genes involved in placental development, in the spermatozoa of partners of women experiencing preeclampsia.
View Article and Find Full Text PDFMethylation and expression of imprinted genes in circulating extracellular vesicles from women experiencing early onset preeclampsia.
Placenta
December 2024
ICMR-National Institute for Research in Reproductive and Child Health (ICMR-NIRRCH), Parel, Mumbai, India. Electronic address:
Introduction: Preeclampsia (PE) is a pregnancy complication marked by high blood pressure, posing risk to maternal and fetal health. "Genomic imprinting", an epigenetic phenomenon regulated by DNA methylation at Differently Methylated Regions (DMR's), influences placental development. Research on circulating extracellular vesicles (EVs) in PE suggests them as potential source for early biomarkers, but methylation status of EV-DNA in Preeclampsia is not reported yet.
View Article and Find Full Text PDFD4Z4 Hypomethylation in Human Germ Cells.
Cells
September 2024
Institute of Human Genetics, Julius Maximilians University, 97074 Wuerzburg, Germany.
Expression of the double homeobox 4 () transcription factor is highly regulated in early embryogenesis and is subsequently epigenetically silenced. Ectopic expression of due to hypomethylation of the D4Z4 repeat array on permissive chromosome 4q35 alleles is associated with facioscapulohumeral muscular dystrophy (FSHD). In peripheral blood samples from 188 healthy individuals, D4Z4 methylation was highly variable, ranging from 19% to 76%, and was not affected by age.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!