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Insights into the Activation and Self-Association of Arrestin-1.
Biochemistry
December 2024
Gavin Herbert Eye Institute - Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, Irvine, California 92697, United States.
Arrestins halt signal transduction by binding to the phosphorylated C-termini of activated G protein-coupled receptors. Arrestin-1, the first subtype discovered, binds to rhodopsin in rod cells. Mutations in , the gene encoding Arrestin-1, are linked to Oguchi disease, characterized by delayed dark adaptation.
View Article and Find Full Text PDFProduction of eukaryotic heliorhodopsins for structural analysis utilizing the LEXSY expression system.
Int J Biol Macromol
December 2024
Institut de Biologie Structurale Jean-Pierre Ebel, Université Grenoble Alpes-Commissariat à l'Energie Atomique et aux Energies Alternatives-CNRS, F-38027 Grenoble, France. Electronic address:
Heliorhodopsins (HeRs) constitute a novel and distinct group of microbial rhodopsins, characterized by the inverted position of C- and N- termini relative to conventional Type I rhodopsins. The production of HeRs for structural and functional investigations has proven challenging, as evidenced by the structural elucidation of only two proteins and the functional characterization of a few others to date. Notably, no eukaryotic HeRs have been reported thus far.
View Article and Find Full Text PDFGenome sequence of an extremely halophilic archaeon isolated from Permian Period halite, Salado Formation in New Mexico, USA: sp. strain NMX12-1.
Microbiol Resour Announc
November 2024
Marine Estuarine Environmental Sciences Program, University of Maryland, College Park, Maryland, USA.
sp. strain NMX12-1, an extremely halophilic Archaeon, was isolated from 250 million-year-old Salado Formation salt crystal in Carlsbad, New Mexico. Single-molecule real-time sequencing revealed a 3.
View Article and Find Full Text PDFThe Impact of Nanobodies on G Protein-Coupled Receptor Structural Biology and Their Potential as Therapeutic Agents.
Mol Pharmacol
September 2024
Gavin Herbert Eye Institute - Center for Translational Vision Research, Department of Ophthalmology (D.S., A.W., K.P.) and Department of Biomedical Engineering (C.C.L.), University of California, Irvine, Irvine, California
The family of human G protein-coupled receptors (GPCRs) comprises about 800 different members, with about 35% of current pharmaceutical drugs targeting GPCRs. However, GPCR structural biology, necessary for structure-guided drug design, has lagged behind that of other membrane proteins, and it was not until the year 2000 when the first crystal structure of a GPCR (rhodopsin) was solved. Starting in 2007, the determination of additional GPCR structures was facilitated by protein engineering, new crystallization techniques, complexation with antibody fragments, and other strategies.
View Article and Find Full Text PDFMolecular basis of CRX/DNA recognition and stoichiometry at the Ret4 response element.
Structure
October 2024
Department of Molecular Physiology and Biophysics, The University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA; Department of Ophthalmology and Visual Sciences, The University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA. Electronic address:
Two retinal transcription factors, cone-rod homeobox (CRX) and neural retina leucine zipper (NRL), cooperate functionally and physically to control photoreceptor development and homeostasis. Mutations in CRX and NRL cause severe retinal diseases. Despite the roles of NRL and CRX, insight into their functions at the molecular level is lacking.
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