AI Article Synopsis
- The study investigates how three mutant strains of pseudorabies virus (PRV), which lack parts of the UL21 gene from herpes simplex virus type 1, behave in various cell types, including SK-6 cells and porcine tissue.
- The M155 mutant produced few infectious particles with little or no DNA, while mutants M133 and M134 generated more complex viral particles, indicating differences in virus assembly.
- All mutants produced C-type particles in nasal mucosa explants, suggesting that the UL21 gene product isn't essential for virus production in porcine tissues, but it likely plays a role in viral DNA packaging.
Article Abstract
We studied the morphogenesis of three pseudorabies virus mutants lacking parts of the gene homologous to the UL21 gene of the herpes simplex virus type 1. The mutants were examined in an SK-6 cell-line, in an SK-6 cell-line expressing the UL21 gene product, in porcine lung alveolar macrophages (PLAM) and in porcine nasal mucosa explants. Although on SK-6 cells and PLAM, the virus-assembly and egress of mutant virus M155, lacking almost the entire UL21 gene, was similar to that of the rescued PRV mutant, M155 producing virions containing little or no DNA (A-type particles). Virus mutants M133 and M134 (lacking 23 and 232 amino acids respectively) produced more C-type particles. In SK-6 cells stably expressing the UL21-encoded protein, all mutants produced C-type particles. All mutants produced C-type particles in nasal mucosa explants, indicating that the UL21-gene product is not essential for virus production in porcine tissue. These results support and extend previous work that indicated a role for the UL21 encoded protein in the packaging of newly replicated viral DNA.
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