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Fluorescent indicators for live-cell and in vitro detection of inorganic cadmium dynamics.
J Fluoresc
July 2022
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, 510006, Guangzhou, People's Republic of China.
Cadmium contamination is a severe threat to the environment and food safety. Thus, there is an urgent need to develop highly sensitive and selective cadmium detection tools. The engineered fluorescent indicator is a powerful tool for the rapid detection of inorganic cadmium in the environment.
View Article and Find Full Text PDFZeta-carbonic anhydrases show CS hydrolase activity: A new metabolic carbon acquisition pathway in diatoms?
Comput Struct Biotechnol J
June 2021
Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy.
CDCA1 is a very peculiar member of the Carbonic Anhydrase (CA) family. It has been the first enzyme to show an efficient utilization of Cd(II) ions in Nature and a unique adaptation capability to live on the surface ocean. Indeed, in this environment, which is extremely depleted in essential metal ions, CDCA1 can utilize Zn(II) or Cd(II) as catalytic metal to support the metabolic needs of fast growing diatoms.
View Article and Find Full Text PDFSputum containing zinc enhances carbapenem resistance, biofilm formation and virulence of Pseudomonas aeruginosa.
Microb Pathog
December 2014
Microbiology Unit, Department of Botany and Plant Biology, Sciences III, University of Geneva, Switzerland. Electronic address:
Pseudomonas aeruginosa chronic lung infections are the leading cause of mortality in cystic fibrosis patients, a serious problem which is notably due to the numerous P. aeruginosa virulence factors, to its ability to form biofilms and to resist the effects of most antibiotics. Production of virulence factors and biofilm formation by P.
View Article and Find Full Text PDFFeedback inhibition by thiols outranks glutathione depletion: a luciferase-based screen reveals glutathione-deficient γ-ECS and glutathione synthetase mutants impaired in cadmium-induced sulfate assimilation.
Plant J
June 2012
Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, La Jolla, California 92093-0116, USA.
Plants exposed to heavy metals rapidly induce changes in gene expression that activate and enhance detoxification mechanisms, including toxic-metal chelation and the scavenging of reactive oxygen species. However, the mechanisms mediating toxic heavy metal-induced gene expression remain largely unknown. To genetically elucidate cadmium-specific transcriptional responses in Arabidopsis, we designed a genetic screen based on the activation of a cadmium-inducible reporter gene.
View Article and Find Full Text PDFImmunoassay for cadmium detection and quantification.
Biomed Environ Sci
June 2009
School of Biosciences & Bioengineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
Objective: To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy (GFAAS) and inductively coupled plasma atomic emission spectroscopy (ICPAES).
Methods: An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated.
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