We have characterized an almond (Prunus dulcis) lipoxygenase (LOX) that is expressed early in seed development. The presence of an active lipoxygenase was confirmed by western blot analysis and by measuring the enzymatic activity in microsomal and soluble protein samples purified from almond seeds at this stage of development. The almond lipoxygenase, which had a pH optimum around 6, was identified as a 9-LOX on the basis of the isomers of linoleic acid hydroperoxides produced in the enzymatic reaction. A genomic clone containing a complete lipoxygenase gene was isolated from an almond DNA library. The 6776-bp sequence reported includes an open reading frame of 4667 bp encoding a putative polypeptide of 862 amino acids with a calculated molecular mass of 98.0 kDa and a predicted pI of 5.61. Almond seed lipoxygenase shows 71% identity with an Arabidopsis LOX1 gene and is closely related to tomato fruit and potato tuber lipoxygenases. The sequence of the active site was consistent with the isolated gene encoding a 9-LOX.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1046/j.1432-1327.2001.02020.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fine-root and leaf acquisitive traits decoupled from chloride accumulation in reflecting the differential salinity tolerance among hybrids.
Front Plant Sci
January 2025
CSIRO, Glen Osmond, Adelaide, SA, Australia.
Improving crop salinity management requires enhanced understanding of salinity responses of leaf and fine-root traits governing resource acquisition, ideally in relation to ion accumulation at intra- or inter-specific levels. We hypothesized that these responses are coupled towards integrated resource conservation for plants under prolonged salt treatment. We tested the hypothesis with a glasshouse experiment on saplings of six contrasting hybrids, subjected to either control or salt treatment (reverse osmosis water versus 3.
View Article and Find Full Text PDFPro-Arg, The Potential Anti-Diabetes Peptide, Screened from Almond by In-Silico Analysis.
Plant Foods Hum Nutr
January 2025
College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing, 404100, China.
Insulin resistance was considered to be the most important clinical phenotype of type 2 diabetes (T2DM). Almond is a widely-consumed nut and long-term intake was beneficial to alleviating insulin resistance in patients with T2DM. Hence, screening of anti-diabetic peptides from almond proteins was feasible based on the effectiveness of peptides in the treatment of T2DM.
View Article and Find Full Text PDFFollowing the commodity risk assessment of and plants for planting from Türkiye, in which (Hemiptera: Diaspididae), the pistachio oyster scale or yellow pistachio scale, was identified as a pest of possible concern, the EFSA Panel on Plant Health performed a pest categorisation for the territory of the European Union (EU). is reported as a polyphagous pest which, however, mainly affects plants of the genus Originating from Asia, it is widely distributed in pistachio producing countries of Central, South and West Asia. Within the EU, the pest has been reported from Cyprus and Greece.
View Article and Find Full Text PDFAlmond Grafting for Plum Pox Virus Resistance Triggers Significant Transcriptomic and Epigenetic Shifts in Peaches.
Int J Mol Sci
December 2024
Department of Plant Breeding, CEBAS-CSIC, Espinardo, P.O. Box 164, 30100 Murcia, Spain.
Sharka disease, caused by the plum pox virus (PPV), negatively impacts stone fruit production, resulting in economic losses. It has been demonstrated that grafting the almond ( (Miller) D.A.
View Article and Find Full Text PDFLipidomics of coconut, almond and soybean milks - Detailed analysis of polar lipids and comparison with bovine milk.
Food Res Int
January 2025
Department of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, Italy.
In this work, the lipidomic analysis on polar components of almond, coconut, and soy beverages was performed by liquid chromatography quadrupole time-of-flight mass spectrometry. A comparison with bovine milk was also performed. A total of 30 subclasses of polar lipids, belonging mainly to glycerophospholipids and sphingolipids, and a total of 572 molecular species were identified.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!