A rapid and efficient method of purification of staphylococcal exfoliative toxin A, the causative agent of staphylococcal scalded skin syndrome (SSSS), has been developed. It is based on ammonium sulfate precipitation of the culture supernatant and hydrophobic interaction chromatography on Phenyl-Sepharose CL-4B. This procedure results in 87-fold purification of this toxin, which appears as a single band in sodium dodecyl sulfate-polyacrylamide gels.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1348-0421.2000.tb02604.x | DOI Listing |
Publication Analysis
Top Keywords
staphylococcal exfoliative
8
exfoliative toxin
8
simple rapid
4
rapid method
4
method purifying
4
purifying staphylococcal
4
toxin rapid
4
rapid efficient
4
efficient method
4
method purification
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!