The comparative evaluation of sensitivity and specificity of the micronucleus and chromosome aberration tests for human lymphocytes at the delayed terms after acute exposure to high-dose as well as during constant exposure to low-dose gamma-radiation has been done. Accordance between these tests registered only in the cases of acute radiation sickness of second and third degrees of severity (irradiation doses above 200 cGy). Unspecificity of micronucleus test for estimation of the radiation load under constant low-intensity irradiation was found.
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J Exp Clin Cancer Res
January 2025
Hepatology Laboratory, Solid Tumors Program, CIMA, CCUN, University of Navarra, Pamplona, Spain.
Background: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive cancer with limited treatment options and a poor prognosis. The critical role of epigenetic alterations such as changes in DNA methylation, histones modifications, and chromatin remodeling, in pancreatic tumors progression is becoming increasingly recognized. Moreover, in PDAC these aberrant epigenetic mechanisms can also limit therapy efficacy.
View Article and Find Full Text PDFPrimary ciliary dyskinesia (PCD, OMIM 244400) is a rare genetic disorder that affects motile cilia and is characterised by impaired mucociliary clearance of the airway epithelium, which results in chronic upper and lower airway infections. While short-read next-generation sequencing technology has been used for the genetic testing of PCD, its effectiveness is limited in identifying variants in the gene because of the nearly identical pseudogene As we confirmed that the gene was not expressed in airway cells, we obtained nasal mucosa biopsy specimens for total RNA sequencing (RNA-seq) with library enrichment using exome oligos. Among the 34 nasal samples from patients suspected of having PCD, three aberrant splicing patterns in were identified in two samples.
View Article and Find Full Text PDFJ Infect Dis
January 2025
Department of Oral and Craniofacial Biology, School of Dentistry, LSU Health New Orleans, USA.
Background: Vulvovaginal candidiasis (VVC), caused primarily by Candida albicans, is currently treated with either prescription or over-the-counter antifungal drugs, often with variable efficacy and relapses. New and improved therapeutic strategies, including drug-free treatment alternatives, are needed. Upon overgrowth or environmental triggers, C.
View Article and Find Full Text PDFCureus
December 2024
Hematology Oncology, Miami Cancer Institute, Miami, USA.
Patients with multiple myeloma (MM) often experience infections due to aberrant immunoglobulin production by malignant plasma cells and immunosuppressive therapeutic interventions that are used to treat the condition. A rare but serious infection that may occur in these patients is Cryptococcus, an encapsulated fungus that typically infects immunocompromised individuals. Cryptococcus infections often present as pneumonia but can disseminate to the central nervous system, potentially causing meningitis.
View Article and Find Full Text PDFToxicol Res
January 2025
Department of Health Functional New Materials, Duksung Women's University, Seoul, 01369 Republic of Korea.
Unlabelled: The aim of this study was to investigate genotoxicity of fucoidan-rich sporophyll (FUPS) using a three-component test battery. Our sulfate analysis method showed that FUPS extract contained 14% fucoidan sulfate. The reverse mutation assay showed that the FUPS extract did not increase the number of revertant colonies in any of the five bacterial strains tested, regardless of metabolic activation by S9 mix.
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