AI Article Synopsis
- PCR amplification and colorimetric identification were utilized to detect DNA of Bartonella henselae and Afipia felis in patients suspected of cat scratch disease.
- Six lymph node biopsies, along with blood and serum samples from 18 patients, were analyzed using specific probes and electrophoresis techniques.
- The results indicated that PCR-DEIA is a sensitive and viable method for diagnosing cat scratch disease, with DNA from Bartonella henselae found in some patient samples.
Article Abstract
Polymerase chain reaction (PCR) amplification and colorimetric identification of amplicons were performed to detect Bartonella henselae and Afipia felis DNA in specimens from patients who were clinically and histologically suspected of having cat scratch disease. PCR products were revealed using 2% ethidium bromide agarose-gel electrophoresis and identified with specific probes in a commercial colorimetric hybridization assay (DEIA) (GEN-ETI-K; DiaSorin, Italy). Six paraffin-embedded lymph node biopsies from 18 patients as well as 18 samples of peripheral whole blood and 18 sera were investigated. Bartonella henselae DNA was recovered from the whole blood of four patients, and Bartonella henselae and Afipia felis DNA were detected in one patient's lymph node biopsy. This study suggests that PCR-DEIA is sufficiently sensitive to be considered feasible for the molecular diagnosis of cat scratch disease.
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| http://dx.doi.org/10.1007/s100960000393 | DOI Listing |
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