Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1385/1-59259-051-9:121 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Hypoxia Microenvironment Preconditioning Attenuated Myocardial Ischemia-Reperfusion Injury via Stc1-Mediating Cardiomyocyte Self-Protection and Neutrophil Polarization.
Adv Sci (Weinh)
December 2024
Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
Ischemic preconditioning (IPC) therapy application to attenuate myocardial ischemia-reperfusion (MI/R) injury in clinical practice remains challenging. The secretome, derived from hypoxia-preconditioned cardiomyocytes (SHPC), potentially mimics the IPC microenvironment and facilitates IPC clinical translation. This study aims to determine whether SHPC can be a feasible alternative to IPC for attenuating MI/R injury, and to identify the functional factor of SHPC.
View Article and Find Full Text PDFStructural basis for the synergetic neutralization of hepatitis E virus by antibody-antibody interaction.
Proc Natl Acad Sci U S A
December 2024
State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Department of Laboratory Medicine, School of Public Health, School of Life Sciences, Xiamen University, Xiamen 361102, China.
Neutralizing antibodies (nAbs) play a crucial role in virology, antibody drug development, and vaccine research. In this study, we investigated the synergistic effect of two hepatitis E virus (HEV) nAbs, 8H3, and 8C11, which have exhibited enhanced neutralizing activity in a rhesus monkey model. We presented crystal structures of 8H3 Fab alone and a triple complex of 8C11 Fab and 8H3 Fab simultaneously binding to the HEV E2s protein (8C11:E2s:8H3).
View Article and Find Full Text PDFStructural insight into recognition of Clostridioides difficile toxin A by novel neutralizing nanobodies targeting QTIN-like motifs within its receptor-binding domain.
Int J Biol Macromol
December 2024
Gamaleya Research Center for Epidemiology and Microbiology, Russian Ministry of Public Health, Moscow 123098, Russia. Electronic address:
Clostridioides difficile causes a large proportion of nosocomial colon infections by producing toxins TcdA and TcdB as key virulence factors. TcdA and TcdB have analogous domain structures with a receptor-binding domain containing C-terminal combined repetitive oligopeptides (CROPs), an attractive target for the development of therapeutic antibodies. Here, we identify and characterize two potent neutralizing single-domain camelid anti-CROPsA antibodies, C4.
View Article and Find Full Text PDFStructure-Based Engineering of Monoclonal Antibodies for Improved Binding to Counteract the Effects of Fentanyl and Carfentanil.
ACS Omega
October 2024
Department of Psychiatry and Behavioral Sciences, School of Medicine, University of Washington, Seattle, Washington 98195, United States.
Article Synopsis
- - The opioid overdose epidemic is worsening due to the prevalence of fentanyl and its analogues in illicit drugs, prompting the need for better treatment options.
- - This study focuses on the isolation and efficacy of two families of monoclonal antibodies (mAbs) designed to target fentanyl and carfentanil, revealing that humanizing these mAbs reduced their effectiveness.
- - Structural analysis identified a key residue, Tyr36, in murine mAbs that is vital for binding to fentanyl and carfentanil, highlighting the significance of structural insights in engineering mAbs for opioid treatment.
Generation and epitope mapping of novel neutralizing monoclonal antibodies against glycoprotein E2 of CSFV.
Int J Biol Macromol
December 2024
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. Electronic address:
Classical swine fever virus (CSFV) is a highly contagious and economically important pathogen threatening pig industry worldwide, the envelope glycoprotein E2 of CSFV is the dominant antigen inducing strong antiviral neutralizing immunity. In this study, 7 monoclonal antibodies (mAbs) with neutralizing potency were generated using E2 protein of CSFV Shimen strain (SM) expressed by eukaryotic cells. Their reactivity with 116 CSFV strains in cell cultures and E2 proteins of 10 subgenotypes in western blots showed different CSFV spectrums they recognized.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!