Auxotrophic markers of B. anthracis strains differing them from other Bacillus representatives have been determined. Chromosome genes from prototrophic B. cereus strain were transduced into auxotrophic B. anthracis strain. The properties of transductants were studied in order to establish common transfer of chromosomal determinants responsible for realization of various signs. Transduction mating between species resulted in construction of prototroph B. anthracis strains (pX01- pX02+), whose derivatives are characterized by decreased virulence for laboratory animals.
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