Steel factor (SLF) plus GM-CSF induces proliferative synergy in myeloid progenitors and factor-dependent cell line MO7e. We previously reported that the protein level of cyclin-dependent kinase inhibitor p21(cip1/waf1) (p21) increased synergistically when MO7e cells were stimulated with SLF plus GM-CSF and that p21 induction was required for SLF synergistic responses. Here we show that this p21 induction is regulated at the transcriptional level. Based on use of a multiprobe RNase protection assay, the synergistic increase of p21 mRNA was unique among many cell cycle regulators. While STAT5A and 5B were activated after stimulation with GM-CSF alone or SLF plus GM-CSF, there was no difference in activation between the groups. p44/42 MAP kinase (ERK1/2) was synergistically activated by SLF plus GM-CSF, but SAPK/JNK and p38 MAP kinase were not. Synergistic induction of p21 was significantly decreased with a MEK1 inhibitor, suggesting that the ERK1/2 pathway is involved in the synergistic increase of p21 after GM-CSF plus SLF stimulation.
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http://dx.doi.org/10.1006/bbrc.2000.4215 | DOI Listing |
J Chem Neuroanat
March 2018
Selcuk U., Faculty of Engineering, Dept. of Electrical and Electronics Engineering, Konya, Turkey.
Neuroimage Clin
March 2018
Neuroimaging Research Unit, Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Scientific Institute, via Olgettina 60, 20132 Milan, Italy; Department of Neurology, Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Scientific Institute, via Olgettina 60, 20132 Milan, Italy. Electronic address:
Med Image Anal
October 2013
Sherbrooke Connectivity Imaging Laboratory (SCIL), Computer Science Department, Université de Sherbrooke, Sherbrooke, Québec, Canada.
We have developed the Tractometer: an online evaluation and validation system for tractography processing pipelines. One can now evaluate the results of more than 57,000 fiber tracking outputs using different acquisition settings (b-value, averaging), different local estimation techniques (tensor, q-ball, spherical deconvolution) and different tracking parameters (masking, seeding, maximum curvature, step size). At this stage, the system is solely based on a revised FiberCup analysis, but we hope that the community will get involved and provide us with new phantoms, new algorithms, third party libraries and new geometrical metrics, to name a few.
View Article and Find Full Text PDFCurr Protoc Immunol
May 2001
Indiana University School of Medicine, Indianapolis, Indiana, USA.
Detection and quantitation of IL-3 and other hematopoietic cytokines in serum samples and culture supernatants has played an important role in understanding their function and pleiotropic properties during the process of hematopoiesis. Several methods for detecting and quantitating cytokines--such as the colony-stimulating factors granulocyte/macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF, also called CSF-1), interleukin 3 (IL-3), and erythropoietin (Epo), as well as two potent costimulating cytokines steel factor (SLF) and Flt3/Flk-2 ligand (Flt3-L)--are described in this unit.
View Article and Find Full Text PDFJ Hematother Stem Cell Res
August 2003
Department of Microbiology and Immunology, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Hematopoietic progenitor cell (HPC) homeostasis is critical in maintaining innate immunity and healing processes. Recently, we demonstrated that Th1 cells regulate HPC homeostasis, partly based on altered homeostasis in Stat4- and Stat6-deficient mice. To explore changes in HPC responsiveness in altered T helper cell environments, we directly examined growth factor-stimulated colony formation and chemokine-induced myelosuppression of HPC in Stat4- and Stat6-deficient bone marrow cells.
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