We have analyzed the total cell extract, cell surface, and secretory protein profiles related to cellular differentiation triggered by dimethylsulfoxide in the insect trypanosomatid Herpetomonas samuelpessoai. The flagellates were cultivated in chemically defined conditions in the absence or in the presence of 4% DMSO, and the resolved protein bands were detected by SDS-PAGE gels and avidin-Western blotting. The cell-associated proteins showed a complex pattern of around 40 silver-staining bands ranging from 15 to 150 kDa. There were generally minor quantitative differences in the protein profile between the non-treated and the DMSO-treated cells. The cell-surface protein profile revealed by the incubation of live parasites with biotin showed a decrease in the expression of the 120 kDa biotinylated polypeptide observed in the DMSO-treated cells when compared with untreated ones. However, control samples of both systems showed an endogenous biotinylated polypeptide of 63 kDa which also presented gelatinolytic activity. The trypanosomatids released at least 10 polypeptides to the culture medium. A low molecular mass exopolypeptide (35 kDa) was found exclusively in untreated cells, whereas a high-molecular-mass exopolypetide (250 kDa) was preferentially found in DMSO-treated cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s002840010188 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Discovery of PPAR Alpha Lipid Pathway Modulators That Do Not Bind Directly to the Receptor as Potential Anti-Cancer Compounds.
Int J Mol Sci
January 2025
Medical Research Core Facility and Platforms (MRCFP)-Drug Discovery Platform, King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs (MNGHA), Riyadh 11481, Saudi Arabia.
Peroxisome proliferator-activated receptors (PPARs) are considered good drug targets for breast cancer because of their involvement in fatty acid metabolism that induces cell proliferation. In this study, we used the KAIMRC1 breast cancer cell line. We showed that the PPARE-Luciferase reporter gets highly activated without adding any exogenous ligand when PPAR alpha is co-transfected, and the antagonist GW6471 can inhibit the activity.
View Article and Find Full Text PDFCurcumin-induced exosomal FTO from bone marrow stem cells alleviates sepsis-associated acute kidney injury by modulating the m6A methylation of OXSR1.
Kaohsiung J Med Sci
December 2024
Department of Emergency Medicine, Affiliated Hospital of Jiangnan University, Wuxi, China.
Curcumin and bone marrow stem cells (BMSCs)-derived exosomes are considered to be useful for the treatment of many human diseases, including sepsis-associated acute kidney injury (SA-AKI). However, the role and underlying molecular mechanism of curcumin-loaded BMSCs-derived exosomes in the progression of SA-AKI remain unclear. Exosomes (BMSCs-EXO or BMSCs-EXO) were isolated from curcumin or DMSO-treated BMSCs, and then co-cultured with LPS-induced HK2 cells.
View Article and Find Full Text PDFThe HR antagonist, JNJ-7777120 treatments ameliorate mild traumatic brain injury by reducing oxidative damage, inflammatory and apoptotic responses through blockage of the ERK1/2/NF-κB pathway in a rat model.
Exp Neurol
December 2024
Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul, Turkiye. Electronic address:
Growing evidence reveals that microglia activation and neuroinflammatory responses trigger cell loss in the brain. Histamine is a critical neurotransmitter and promotes inflammatory responses; thus, the histaminergic system is a potential target for treating neurodegenerative processes. JNJ-7777120, a histamine H4 receptor (HR) antagonist, has been shown to alleviate inflammation, brain damage, and behavioral deficits effectively, but there is no report on its role in brain trauma.
View Article and Find Full Text PDFUltra-fast vitrification and rapid elution of human oocytes: part I. germinal vesicle model validation.
Reprod Biomed Online
December 2024
California Fertility Partners/Pinnacle Fertility, Los Angeles, CA 90025, USA.
Research Question: Can GV-oocytes serve as an effective model to test the efficacy of ultra-fast vitrification (UFV)/ rapid elution (RE) treatments to support reliable, high survival rates and sustained functionality?
Design: Prospective pilot cohort studies were performed to investigate the feasibility of non-equilibration, UFV to sustain cellular integrity and development in contrast to control vitrification (CV: 10-15min ES/ 1min VS). In Phase 1, we applied a 2 × 2 factorial design (n=25-30 eggs/group) to evaluate post-warming dilution treatments: conventional multi-step (CD) versus rapid elution (RE; one-step), including an apriori fresh egg control group. Phase 1/2 focused on survival and maturation assessments, including meiotic spindle formation (Phase 2).
Single cell spatial profiling of FFPE splenic tissue from a humanized mouse model of HIV infection.
Biomark Res
October 2024
MRL, Merck & Co., Inc, Rahway, NJ, USA.
Background: Latency remains a major obstacle to finding a cure for HIV despite the availability of antiretroviral therapy. Due to virus dormancy, limited biomarkers are available to identify latent HIV-infected cells. Profiling of individual HIV-infected cells is needed to explore potential latency biomarkers and to study the mechanisms of persistence that maintain the HIV reservoir.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!