Receptor analysis using the radioligand binding saturation method in situations requiring a large concentration range of the ligand is theoretically straightforward but in practice can be relatively difficult. In this paper we review three approaches for carrying out such experiments and assess the strengths and weaknesses of each. The three are two saturation experiments, two homologous competition experiments, and the mixed homologous saturation experiment. The best approach depends upon the experimental conditions and constraints of the system studied, but in general the mixed homologous saturation experiment appears to be the most effective at minimizing the amount of radioligand needed while providing the greatest reliability in the results.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0024-3205(00)00877-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Anti-Müllerian hormone levels in relation to ovarian structures, season and age in Lipizzaner broodmares.
Acta Vet Hung
January 2025
7Department of Precision Animal Breeding and Livestock Biotechnology, Institute of Animal Sciences, Hungarian University of Agriculture and Life Sciences, Herceghalom, Hungary.
The anti-Müllerian hormone (AMH) is a granulosa cell-derived hormone that has been associated with female fertility and reflects the population of growing follicles. This study aimed to evaluate the average concentration of AMH in Lipizzaner mares, as well as to determine the relationship between AMH concentration and follicle number and size. We also investigated the relationship between the age of mares and their AMH levels.
View Article and Find Full Text PDFThe effect of selective surface interaction on polymer phase separation with explicit polydispersity during polymerization.
Soft Matter
January 2025
Department of Chemical and Biomolecular Engineering, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
In polymerization-induced phase separation, the impact of polymer-substrate interaction on the dynamics of phase separation for polymer blends is important in determining the final morphology and properties of polymer materials as the surface can act as another driving force for phase separation other than polymerization. We modify the previously-developed polymerizing Cahn-Hilliard (pCH) method by adding a surface potential to model the phase separation behavior of a mixture of two species independently undergoing linear step-growth polymerization in the presence of a surface. In our approach, we explicitly model polydispersity by separately considering different molecular-weight components with their own respective diffusion constants, and with the surface potential preferentially acting on only one species.
View Article and Find Full Text PDFThin film microextraction of PAHs from wastewater samples using an oxine modified iron mesh followed by dispersive liquid-liquid microextraction prior to gas chromatography analysis.
Anal Methods
January 2025
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
In the present research, an attempt has been made to develop a new thin film microextraction method for the extraction of several polycyclic aromatic hydrocarbons from aqueous samples collected from different industrial units prior to their analysis by gas chromatography combined with a flame ionization detector. In this approach, a thin iron mesh was modified by the formation of iron(II) oxinate on its surface and used for the extraction of analytes without an additional sorbent. For this purpose, first, the mesh was immersed in a sulfuric acid solution and then transferred into an 8-hydroxy quinoline (oxine) solution dissolved in ammonia solution.
View Article and Find Full Text PDFAbrupt transformation of west Greenland lakes following compound climate extremes associated with atmospheric rivers.
Proc Natl Acad Sci U S A
January 2025
Department of Aquatic Ecology, Netherlands Institute of Ecology, Wageningen 6708 PB, The Netherlands.
Arctic ecosystems are affected by accelerated warming as well as the intensification of the hydrologic cycle, yet understanding of the impacts of compound climate extremes (e.g., simultaneous extreme heat and rainfall) remains limited, despite their high potential to alter ecosystems.
View Article and Find Full Text PDFThe Plant Ionome as a Functional Trait: Variation across Bioclimatic Regions and Functional Groups.
Physiol Plant
January 2025
School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Israel.
Plant chemical composition is a trait gaining increasing importance in plant ecology. However, there is limited research on the patterns and drivers of its variation among different plant functional groups and bioclimatic regions. We conducted an analysis of ionomes utilising X-ray fluorescence on 83 plant species from four distinct functional groups (grasses, legumes, forbs and woody species); we marked plots across 15 sites located in both the desert and Mediterranean bioclimatic regions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!