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Whole genome sequencing revealed high occurrence of antimicrobial resistance genes in bacteria isolated from poultry manure.

Int J Antimicrob Agents

January 2025

Department of Botany, Institute of Science, Banaras Hindu, University, Varanasi, Uttar Pradesh, 221005, India. Electronic address:

Global demand for food has driven expansion and intensification of livestock production, particularly in developing nations where antibiotic use is often routine. Waste from poultry production, including manure, is commonly utilized as fertilizers in agroecosystems, risking environmental contamination with potentially zoonotic bacteria and antimicrobial resistance genes (ARGs). Here, 33 bacterial isolates were recovered from broiler (n=17) and layer (n=16) chicken manure by aerobic culture using Luria Bertani agar.

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Purpose: This study aimed to evaluate the prognosis of the not evaluated (NE) group by comparing it with the lost to follow-up (LTFU) group among patients with multidrug/rifampin-resistant tuberculosis (MDR/RR-TB).

Materials And Methods: This was a retrospective longitudinal follow-up study using an integrated database constructed by data linkage of the three national databases. This database included 7226 cases of MDR/RR-TB notified between 2011 and 2017 in South Korea.

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Broth microdilution protocol for determining antimicrobial susceptibility of Legionella pneumophila to clinically relevant antimicrobials.

J Microbiol Methods

January 2025

Medical Microbiology, Division of Infection and Immunity, Cardiff University, Cardiff, UK; UK Health Security Agency, Bacteriology Reference Department, London, UK. Electronic address:

Currently there is no detailed, internationally agreed protocol defined to evaluate antimicrobial susceptibility testing (AST) for Legionella pneumophila (required to establish epidemiological cut-off value or "ECOFF" boundaries); therefore, antimicrobial resistance in these isolates cannot be defined. AST methods utilising media containing activated charcoal as an ingredient, to enable Legionella growth, are unreliable as noted in an internationally authored opinion paper and a new gold standard is required. Here we define a detailed protocol for broth microdilution (BMD) using defined cell culture collection-deposited control reference strains (Philadelphia-1 and Knoxville-1) as well as two accessible reference strains with moderately (lpeAB-carrying) and markedly (23S rRNA mutation-carrying) elevated azithromycin minimum inhibitory concentration (MIC).

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First report of causing Diplodia leaf streak disease of maize in Yunnan Province, China.

Plant Dis

December 2024

Yunnan Agricultural University College of Plant Protection, , Yunnan Agricultural University, Fengyuan Road 95, Kunming, kunming, China, 650201.

Maize (Zea mays. L) is cultivated globally as a staple food crop, animal feed, and biofuel. However, persistent diseases in maize have led significant yield losses and a decline in grain quality (Yang et al.

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Povidone-iodine and silver nitrate are equally effective in eradicating staphylococcal biofilm grown on a titanium surface: an in-vitro analysis.

J Hosp Infect

January 2025

'Sir John Charnley' Hip Surgery Unit, Institute of Orthopaedics 'Carlos E. Ottolenghi', Italian Hospital of Buenos Aires, Buenos Aires, Argentina. Electronic address:

Article Synopsis
  • The study investigates which antibacterial solutions are most effective in eradicating biofilms formed by staphylococcal bacteria on titanium surfaces during DAIR surgery.
  • Five antibacterial agents are tested: povidone-iodine, rifampicin, silver nitrate, copper sulphate, and chlorhexidine, focusing on their minimum biofilm eradication concentrations (MBEC) against both MSSA and MRSA strains.
  • Results indicate that povidone-iodine and silver nitrate are significantly more effective, achieving at least 99.9% reduction in biofilm, compared to others like rifampicin and copper sulphate, which showed limited effectiveness.
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