PseudoBase: structural information on RNA pseudoknots.

Nucleic Acids Res

Group Theoretical Biology and Phylogenetics, Institute of Evolutionary and Ecological Sciences, Leiden University, Kaiserstraat 63, 2311GP Leiden, The Netherlands.

Published: January 2001

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In alternate organic synthesis, biocatalysis using enzymes provides a more stereoselective and cost-effective approach. Synthesis of unnatural nucleosides by nucleoside base exchange reactions using nucleoside-metabolizing enzymes has previously shown that the 5-position recognition of pyrimidine bases on nucleoside substrates is loose and can be used to introduce functional molecules into pyrimidine nucleosides. Here we explored the incorporation of purine pseudo bases into nucleosides by the base exchange reaction of pyrimidine nucleoside phosphorylase (PyNP), demonstrating that an imidazole five-membered ring is an essential structure for the reaction.

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Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106.

Cell Rep

January 2023

European Molecular Biology Laboratory, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France. Electronic address:

The antiviral pseudo-base T705 and its de-fluoro analog T1106 mimic adenine or guanine and can be competitively incorporated into nascent RNA by viral RNA-dependent RNA polymerases. Although dispersed, single pseudo-base incorporation is mutagenic, consecutive incorporation causes polymerase stalling and chain termination. Using a template encoding single and then consecutive T1106 incorporation four nucleotides later, we obtained a cryogenic electron microscopy structure of stalled influenza A/H7N9 polymerase.

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Background: We study in this work the inverse folding problem for RNA, which is the discovery of sequences that fold into given target secondary structures.

Results: We implement a Lévy mutation scheme in an updated version of aRNAque an evolutionary inverse folding algorithm and apply it to the design of RNAs with and without pseudoknots. We find that the Lévy mutation scheme increases the diversity of designed RNA sequences and reduces the average number of evaluations of the evolutionary algorithm.

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Background: RNA pseudoknot structures play an important role in biological processes. However, existing RNA secondary structure prediction algorithms cannot predict the pseudoknot structure efficiently. Although random matching can improve the number of base pairs, these non-consecutive base pairs cannot make contributions to reduce the free energy.

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RNAknot: A new algorithm for RNA secondary structure prediction based on genetic algorithm and GRASP method.

J Bioinform Comput Biol

October 2019

Computer Science Department, MACS Lab, Faculty of Science, Moulay Ismail University, Meknes, BP 11201, Morocco.

The prediction of the optimal secondary structure for a given RNA sequence represents a challenging computational problem in bioinformatics. This challenge becomes harder especially with the discovery of different pseudoknot classes, which is a complex topology that plays diverse roles in biological processes. Many recent studies have been proposed to predict RNA secondary structure with some pseudoknot classes, but only a few of them have reached satisfying results in terms of both complexity and accuracy.

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