An intraluminal membrane oxygenator (IMO) prototype was surgically inserted in the ileum and evaluated as a method of supporting systemic oxygenation in an acutely hypoxemic porcine model. Animals were assigned randomly to the test (n = 12) or the control (n = 8) groups, which underwent identical protocols with the exception of the O2 flow in the IMO device, which was shut off in the control group. In each case, hypoxia was induced by a reduction in the inspired oxygen fraction (FiO2) to 0.14. A highly significant improvement (p < 0.005) in arterial and venous O2 content and lower arteriovenous O2 difference (p < 0.05), cardiac output, and hemoglobin (p < 0.005) were found in the test group during hypoxia. The results show that it is possible to meet a physiologically significant portion of the body's O2 demands via the intestine during respiratory hypoxia and suggests that similar devices may be of significant potential value as a supplemental oxygenation device in cases of respiratory distress.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1046/j.1525-1594.2000.06563.x | DOI Listing |
Trends Plant Sci
January 2025
University of Vienna, Djerassiplatz 1, 1030 Vienna, Austria; University of Applied Sciences Dresden, Pillnitzer Platz 2, 01326 Dresden, Germany. Electronic address:
Recent research indicates an involvement of microautophagy in the uptake of seed storage proteins (SSPs) into the plant-specific protein storage vacuole (PSV), particularly in cereal grains. However, because microautophagy plays a vital role in cellular homeostasis by degrading and recycling cellular components, we question whether it is a suitable term for a process involved in long-term storage. Additionally, because fission-type microautophagy shares mechanistic similarities with the intraluminal vesicle (ILV) formation of multivesicular bodies (MVBs), we draw parallels between microautophagy and membrane remodeling facilitated by the endosomal sorting complex required for transport (ESCRT).
View Article and Find Full Text PDFBone
January 2025
Department of Preventive and Restorative Dental Sciences, School of Dentistry, University of California at San Francisco, San Francisco, CA, United States of America.
This paper presents a review of the potential role of the endoplasmic reticulum/Golgi complex and intracellular vesicles in mediating events leading to or associated with vertebrate tissue mineralization. The possible importance of these organelles in this process is suggested by observations that calcium ions accumulate in the tubules and lacunae of the endoplasmic reticulum and Golgi. Similar levels of calcium ions (approaching millimolar) are present in vesicles derived from endosomes, lysosomes and autophagosomes.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Department of Animal Physiology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland.
The aim of this study was to elucidate the impact of porcine pancreatic enzymes (Creon pancrelipase) in comparison to microbial-derived alpha amylase (MD amylase) on the small intestine wall structure, mucosal glycogen accumulation, and enterocyte turnover. The impact of enzyme supplementation on the small intestine was explored in 18 pigs with surgically induced exocrine pancreatic insufficiency (EPI). Four healthy pigs served as the control group.
View Article and Find Full Text PDFJ Extracell Vesicles
January 2025
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China.
Japanese encephalitis virus (JEV) is a neurotropic zoonotic pathogen that poses a serious threat to public health. Currently, there is no specific therapeutic agent available for JEV infection, primarily due to the complexity of its infection mechanism and pathogenesis. Extracellular vesicles (EVs) have been known to play an important role in viral infection, but their specific functions in JEV infection remain unknown.
View Article and Find Full Text PDFSci Adv
January 2025
Department of Biochemistry Cell and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
Lysosomal pH dysregulation is a critical element of the pathophysiology of neurodegenerative diseases, cancers, and lysosomal storage disorders (LSDs). To study the role of lysosomes in pathophysiology, probes to analyze lysosomal size, positioning, and pH are indispensable tools. Here, we developed and characterized a ratiometric genetically encoded lysosomal pH probe, RpH-ILV, targeted to a subpopulation of lysosomal intraluminal vesicles.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!