Randomly amplified polymorphic DNA (RAPD) analysis was performed for the molecular genetic typing of 30 Salmonella enterica subsp. enterica strains isolated from chickens and ducks in Thailand. Six different primers were tested for their discriminatory ability. While some of the primers could only differentiate between the different serovars, the use of multiple primers showed that the RAPD method could also subdivide within a given serovar. The Ready-To-Go RAPD analysis beads used, resulted in reproducible and stable banding patterns. As the RAPD technique is simple, rapid and rather cheap, we suggest that it may be a valuable new tool for studying the molecular genetic epidemiology of S. enterica ssp. enterica, both inter- and intra-serovars.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1574-695X.2000.tb01526.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Rapid Adaptation to Prevent Drug Use (RAPD): protocol of a pilot randomized trial to enhance the impact of an evidence-based intervention for youth.
Pilot Feasibility Stud
January 2025
Center for Healthcare Organization and Implementation Research, VA , Boston Healthcare System, 150 South Huntington Avenue, Boston, 02130, USA.
Background: Drug use trends change rapidly among youth, leaving intervention experts struggling to respond promptly. Delays in responses can lead to preventable morbidity and mortality. The COVID-19 pandemic underscored the need for implementation science to facilitate rapid, equitable responses using existing treatment and prevention efforts.
View Article and Find Full Text PDFHighly Potent New Probiotic Strains from Traditional Turkish Fermented Foods.
Curr Microbiol
January 2025
Department of Nanotechnology Engineering, Abdullah Gul University, Kayseri, Türkiye.
Traditional Turkish fermented foods like boza, pickles, and tarhana are recognized for their nutritional and health benefits, yet the probiotic potential of lactic acid bacteria (LAB) strains isolated from them remains underexplored. Sixty-six LAB strains were isolated from fermented foods using bacterial morphology, Gram staining, and catalase activity. The isolates were differentiated at strain level by RAPD-PCR (Random Amplification of Polymorphic DNA-Polymerase Chain Reaction) and twenty-five strains were selected for further evaluation of acid and bile salt tolerance.
View Article and Find Full Text PDF<b>Background and Objective:</b> Black soybeans [<i>Glycine max</i> (L.) Merr] are among the important crops, but the cultivated resources are normally low-yielding, susceptible to diseases and low profit. Therefore, it is necessary to evaluate the genetic diversity of black soybean germplasms for breeding programs.
View Article and Find Full Text PDFConstruction of a fusant bacterial strain simultaneously degrading atrazine and acetochlor and its application in soil bioremediation.
Sci Total Environ
January 2025
Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of Chin), Gongzhuling 136100, Jilin, China. Electronic address:
Application of herbicide-degrading bacteria is an effective strategy to remove herbicide in soil. However, the ability of bacteria to degrade a herbicide is often severely limited in the presence of other pesticide. In this study, the atrazine-degrading strain Klebsiella varicola FH-1 and acetochlor-degrading strain Bacillus Aryabhatti LY-4 were used as parent strains to construct the recombinant RH-92 strain through protoplast fusion technology.
View Article and Find Full Text PDFAssessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis.
Ann Clin Microbiol Antimicrob
January 2025
Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Proteus mirabilis (P. mirabilis) is one of the most important causative pathogens associated with complicated urinary tract infections with a 20% incidence. For epidemiological determinations, several phenotypic and molecular typing methods have been implicated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!