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Mitochondria-targeting nanostructures from enzymatically degradable fluorescent amphiphilic polyesters.
Nanoscale
January 2025
School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science (IACS), 2A and 2B Raja. S. C. Mullick Road, Jadavpur, Kolkata 700032, India.
Water-soluble π-conjugated luminescent bioprobes have been broadly used in biomedical research but are limited by the nonbiodegradability associated with their rigid C-C backbones. In the present work, we introduced three naphthalene monoimide (NMI)-functionalized amphiphilic fluorescent polyesters (P1, P2, and P3) prepared by transesterification of functional diols with an activated diester monomer of adipic acid. These polyesters featured a side-chain NMI fluorophore, imparting the required hydrophobicity for self-assembly in water and endowing the polymeric nanoassemblies with green fluorescence.
View Article and Find Full Text PDFPrevention of refeeding syndrome: Evaluation of an enteral refeeding protocol for severely undernourished children.
J Pediatr Gastroenterol Nutr
January 2025
Hospices Civil de Lyon, Hôpital Femme Mère Enfant, Service de Gastroentérologie, Hépatologie et Nutrition Pédiatriques, Université Claude Bernard Lyon-1, CarMeN Laboratory, INRAE, UMR1397, INSERM, UMR1060, Pierre-Bénite, France.
Objectives: Refeeding syndrome (RS) defines the deleterious clinical and metabolic changes occurring during nutritional support of severely malnourished patients. Pediatric guidelines to prevent and treat RS are scarce and highly variable. This study aimed to evaluate the effectiveness and safety of an enteral refeeding protocol in severely undernourished hospitalized children with anorexia nervosa (AN) or organic diseases (OD).
View Article and Find Full Text PDFSignificance of KLK7 expression, polymorphisms, and function in sheep horn growth.
BMC Genomics
January 2025
State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Background: Sheep horns play a critical role in the survival and reproduction of sheep. Research on sheep horns not only aids in comprehending their biological roles but is also vital for developing hornless breeds. Although previous studies have suggested that KLK7 may be associated with keratin growth, there are few studies that have focused on the role of KLK7 in sheep horns.
View Article and Find Full Text PDFCharacterization of insulin and bile acid complexes in liposome by different mass spectrometry techniques.
Anal Bioanal Chem
January 2025
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Insulin bound with ligand molecules can improve its bioavailability in oral formulations. In this work, the interactions between insulin and bile acids of taurocholic acid (TCA) and glycocholic acid (GCA) are characterized using different mass spectrometry (MS) methods. Electrospray (ESI)-MS analysis revealed that GCA and TCA could interact with insulin individually or together through non-covalent bonds, and the products included mGCA-insulin, nTCA-insulin, and mGCA-nTCA-insulin complexes.
View Article and Find Full Text PDFA-D-A type fluorescent probe with dual quaternary-ammonium-salt anchors for turn on detection of HSA in wide emission gamut.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122 China. Electronic address:
Human serum albumin (HSA) is a key protein implicates in various physiological and pathological conditions such as renal injury, diabetes mellitus. Herein, we report an AIE-active fluorescent probe (DNI-4) for detection of HSA with a "turn on" response covering visible and near-infrared region (500 - 800 nm). Combining with a triphenylamine and two 1,8-naphthalimide moieties, the chromophore segment of DNI-4 forms a "A-D-A" type molecular architecture with the twisted intramolecular charge transfer property.
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