A method is described for the monolayer cultivation of primary cell suspensions and established cell lines directly in carbon-coated BEEM capsules, BEEM capsules are routinely employed by electron microscopists in tissue embedding procedures; growing monolayer cultures directly on the lids of inverted BEEM capsules presents the obvious advantage of maintaining cell to cell and cell to substratum conthaets with a minimum of stress and damage in the preparative steps for electron microscopy.
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http://dx.doi.org/10.1007/BF02832795 | DOI Listing |
Eur J Morphol
December 2001
Department of Histology & Embryology, Uludag University Faculty of Medicine, Bursa, Turkey.
Successful results of microwave polymerisation of different epoxy formulations have been reported in the literature. The present study was intended to shorten the time needed for polymerisation of epoxy resin by the use of a microwave technique. A standard double fixation and tissue processing was applied to samples of rat kidney tissue.
View Article and Find Full Text PDFJ Microsc
August 2001
Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
The use of microwave irradiation (MWI) to accelerate fixation, dehydration and contrasting (staining) for electron microscopy has been applied to the development of rapid methods to process biological samples in electron microscopy. A simple explanation is that the reduced time in those procedures is due to heating. In this paper we propose a contrasting method for thin sections that avoids the thermal effects of MWI.
View Article and Find Full Text PDFNoshuyo Byori
April 1995
Department of Neurosurgery, Miyazaki Medical College.
The means of examination of small-amount samples by transmission electron microscopy (TEM) is described. In this study, the amount of cells necessary for TEM was examined by using a KMU-100 cultured cell line. Floating cells diluted with phosphate buffer solution (PBS) to several concentrations, were gathered in the tip of Beem capsules by means of centrifuge.
View Article and Find Full Text PDFMicrosc Res Tech
December 1993
Mastology Research Institute, Baton Rouge, Louisiana 70816.
A simplified technique for the monolayer growth of cultured cells and their in situ embedment on the inner surface of the pyramidal portion of the Beem capsule for electron microscopy has been developed. The results demonstrated that the cell monolayers grew well on the surface of the Beem capsule and could be embedded in situ. Electron micrographs showed cells in their natural state of contact with one another.
View Article and Find Full Text PDFScanning Microsc
June 1991
Department of Ultrastructural Pathology, Walter Reed Army Institute of Research, Washington, DC 20307.
A simple technique for processing cells grown on BEEM capsule caps for scanning electron microscopic (SEM) observation is described. The cells adhere to a substratum on the inner surface of caps coated with either an egg white-polyvinylpyrrolidone solution or a polylysine solution in the case of eukaryotic cells and an agar solution in the case of bacteria. The adhering cells are cultured and then processed in situ for SEM observation.
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