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Recent studies have demonstrated that the production of bidirectional enhancer-derived transcripts (eRNAs) is a characteristic of an active Cis-regulatory element (CRE). Higher levels of eRNA synthesis correlate with the activation of histone modifications, a potentially valuable tool for deciphering the complexity of the gene regulatory network. To understand the changes of CREs during gonadal development in mice, we collected gonadal WT1-positive cells from the piggyBac-Wt1-mCherry-2A-EGFP (PBWt1-RG) reporter strain at E13.
View Article and Find Full Text PDFEffectiveness of Voytik-Harbin Protocol in Fabrication of Ram's Testicular-Derived Hydrogel and Its Impact on Mouse Spermatogenesis.
Int J Fertil Steril
January 2025
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Background: The utilization of decellularized extracellular matrix (dECM) derived from animal testis tissue has demonstrated potential as a component of tissue-specific scaffolds. Current research is mostly centered around dECM as a natural resource for culturing testicular cells. This study aimed to assess firstly the comparison of Voytik-Harbin (VH) and Frytes protocol in creating Ram's dECM testis hydrogel and secondly the evaluation of the best protocol effect on spermatogenesis.
View Article and Find Full Text PDFDuck Tembusu virus induced mitophagy in vacuolate spermatogenic cells is mediated by PINK1-Parkin pathway.
Poult Sci
January 2025
Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China. Electronic address:
As a significant emerging and re-emerging pathogen in China, the widely spread of Duck Tembusu virus (DTMUV) caused enormous economic losses to poultry industry. On account of DTMUV diseases' main symptoms on haemorrhagic oophoritis, intensive attentions were focused on female reproductive organ. Nevertheless, the DTMUV infection of sperm and testis manifested that testis was an important vector for vertical transmission of DTMUV.
View Article and Find Full Text PDFNon-neoplastic and neoplastic scrotal pathologies on magnetic resonance imaging: a pictorial essay.
Radiol Bras
January 2025
Hospital Moinhos de Vento, Porto Alegre, RS, Brazil.
Magnetic resonance imaging is an essential tool for the assessment of the scrotum, particularly in cases with inconclusive ultrasound findings. It has a great capacity to differentiate between intratesticular and extratesticular lesions, as well as between neoplastic and non-neoplastic lesions. By providing an accurate characterization of lesions, magnetic resonance imaging plays a crucial role in preoperative tumor staging and decision-making.
View Article and Find Full Text PDFA nomogram for predicting survival in patients with primary testicular lymphoma: A population-based study.
Urol Oncol
January 2025
Department of Oncology, The Fifth Medical Center, Chinese PLA (People's Liberation Army) General Hospital, Beijing, China. Electronic address:
Background: Primary testicular lymphoma (PTL) is a rare malignancy whose epidemiology and prognosis have not been studied.
Materials And Methods: PTL patient data were collected from the SEER online database, and the data were divided into a training cohort and a validation cohort according to random assignment. The training cohort was subjected to a one-way COX regression analysis, and statistically significant differences were included in the multi-factor COX regression analysis and constructed nomograms.
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