Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Monocyte chemotactic protein-1 (MCP-1) chemoattracts and activates monocytes. The nature of the genes that are transcriptionally activated in the monocytes by MCP-1 is not well understood. To identify such genes, human blood monocytes were incubated with or without MCP-1 for periods of 1, 4, and 12 h and the RNA extracted from these monocytes was subjected to differential display. The differentially expressed transcripts were cloned and sequenced. Differential display showed that interleukin-1beta (IL-1beta) gene expression was upregulated by MCP-1 treatment of monocytes for 4 to 12 h. Quantitative PCR and ELISA assays showed that MCP-1 treatment caused elevation in the levels of IL-1beta transcripts and protein, respectively. Immunoblot analysis showed that most of the protein was pro-IL-1beta. Since IL-1beta is known to induce MCP-1 synthesis, the present demonstration that MCP-1 induces IL-1beta synthesis suggests that the induction of each other would amplify the biological effects of these cytokines during inflammation.
Download full-text PDF |
Source |
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http://dx.doi.org/10.1006/bbrc.2000.3619 | DOI Listing |
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