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Development of translationally active cell lysates from different filamentous fungi for application in cell-free protein synthesis.
Enzyme Microb Technol
January 2025
Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, Senftenberg 01968, Germany. Electronic address:
There is an enormous potential for cell-free protein synthesis (CFPS) systems based on filamentous fungi in view of their simple, fast and mostly inexpensive cultivation with high biomass space-time yields and in view of their catalytic capacity. In 12 of the 22 different filamentous fungi examined, in vitro translation of at least one of the two reporter proteins GFP and firefly luciferase was detected. The lysates showing translation of a reporter protein usually were able to synthesize a functional cell-free expressed unspecific peroxygenase (UPO) from the basidiomycete Cyclocybe (Agrocybe) aegerita.
View Article and Find Full Text PDFInvestigating the LiO-AlO-BO matrix: A promising approach for immobilizing radioactive waste.
Appl Radiat Isot
January 2025
Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81300, Skudai, Johor, Malaysia.
Dealing with radioactive waste, particularly from various industrial processes, poses significant challenges. This paper explores the use of lithium aluminate borate (Li-Al-B) glass matrix as an alternative method for immobilizing radioactive waste, focusing specifically on waste generated in tin smelting industries, known as tin slag. The study primarily concentrates on transforming tin slag, a byproduct abundant in Natural Occurring Radioactive Material (NORM), into a stable and safe form for disposal.
View Article and Find Full Text PDFTargeted FTO knockout in endothelial cells Boosts adhesion and lowers inflammatory infiltration to alleviate pulmonary arterial hypertension.
Biochem Biophys Res Commun
January 2025
Department of Ultrasonography, Fuwai Yunnan Hospital, Chinese Academy of Medical, Sciences/Affiliated Cardiovascular Hospital of Kunming Medical University, Kunming, 650102, China. Electronic address:
Pulmonary arterial hypertension (PAH) is a syndrome characterized by increased pulmonary vascular resistance and elevated pulmonary artery pressure, ultimately leading to right heart failure and even death. Increasing evidence implicates the fat mass and obesity-associated protein (FTO) in various metabolic and inflammatory pathways; however, its role in pulmonary endothelial function and PAH remains largely unexplored. In this study, we examined the effects of endothelial cell-specific FTO knockout on PAH development.
View Article and Find Full Text PDFFull fine-tuning strategy for endoscopic foundation models with expanded learnable offset parameters.
Biomed Phys Eng Express
January 2025
Shandong Normal University, Jinan, Jinan, Shandong, 250014, CHINA.
In the medical field, endoscopic video analysis is crucial for disease diagnosis and minimally invasive surgery. The Endoscopic Foundation Models (Endo- FM) utilize large-scale self-supervised pre-training on endoscopic video data and leverage video transformer models to capture long-range spatiotemporal dependencies. However, detecting complex lesions such as gastrointestinal metaplasia (GIM) in endoscopic videos remains challenging due to unclear boundaries and indistinct features, and Endo-FM has not demonstrated good performance.
View Article and Find Full Text PDFLeveraging Artificial Intelligence to Develop a Decision-Support Tool for Visual Symptom Assessment of Grapevine Leafroll and Red Blotch Diseases.
Plant Dis
January 2025
University of California Davis, Cooperative Extension, Napa, California, United States;
The timely detection of viral pathogens in vineyards is a critical aspect of management. Diagnostic methods can be labor-intensive and may require specialized training or facilities. The emergence of artificial intelligence (AI) has the potential to provide innovative solutions for disease detection but requires a significant volume of high-quality data as input.
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