The 5'-flanking region of the human FcRn alpha-chain gene was analyzed for its ability to directly express the chloramphenicol acetyltransferase (CAT) reporter gene in NIH3T3 and Lu106 cells. Transient transfection of the CAT constructs revealed that there was promoter activity in the region -660 to +300 of the 5'-flanking sequence. Electrophoretic mobility-shift assays showed that there are functional binding sites for Sp1 or Sp1-like factors, AP1 or a related factor, and additional unidentified proteins in the promoter region.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0167-4781(00)00068-3 | DOI Listing |
Publication Analysis
Top Keywords
promoter region
8
region human
8
human fcrn
8
analysis promoter
4
region
4
fcrn gene
4
gene 5'-flanking
4
5'-flanking region
4
fcrn alpha-chain
4
alpha-chain gene
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!