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The [(bathophenanthroline):Fe] complex as an aromatic non-polymeric medium for purification of human lactoferrin.
J Chromatogr A
September 2024
Department of Chemical Sciences, Ariel University, 70400, Ariel, Israel. Electronic address:
We describe a non-chromatographic, ligand-free platform for the efficient purification of recombinant human lactoferrin (LF). The platform consists of a [metal:chelator] complex precipitate in the presence of osmotically active polyethylene glycol 6000 (PEG-6000). Purification is achieved in three stages.
View Article and Find Full Text PDFPolymeric Materials in Speciation Analysis Based on Solid-Phase Extraction.
Molecules
December 2023
Faculty of Chemistry and Pharmacy, University of Sofia "St. Kliment Ohridski", 1, James. Bourchier Blvd.1, 1164 Sofia, Bulgaria.
Speciation analysis is a relevant topic since the (eco)toxicity, bioavailability, bio (geo)chemical cycles, and mobility of a given element depend on its chemical forms (oxidation state, organic ligands, etc.). The reliability of analytical results for chemical species of elements depends mostly on the maintaining of their stability during the sample pretreatment step and on the selectivity of further separation step.
View Article and Find Full Text PDFEnantioselective and Chemoselective Optical Detection of Chiral Organic Compounds without Resorting to Chromatography.
Chem Asian J
December 2023
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India.
Enantiorecognition and resolution are of essential importance in many diverse areas of science. Whenever there arises a need to analyze/investigate enantiomers in different situations chromatography stands up in our minds immediately. Nevertheless, chemoselective and enantioselective recognition/discrimination (without going for separation) constitutes a different perception and requirement.
View Article and Find Full Text PDFInsulin purification-Innovation continuum via synthesis of fundamentals, technology, and modeling.
Biotechnol Bioeng
August 2024
Process R&D, Merck & Co., Inc, Rahway, New Jersey, USA.
Advancement in all disciplines (art, science, education, and engineering) requires a careful balance of disruption and advancement of classical techniques. Often technologies are created with a limited understanding of fundamental principles and are prematurely abandoned. Over time, knowledge improves, new opportunities are identified, and technology is reassessed in a different light leading to a renaissance.
View Article and Find Full Text PDFConjugated detergent micelles as a platform for IgM purification.
Biotechnol Bioeng
July 2022
Department of Chemical Sciences, Ariel University, Ariel, Israel.
Immunoglobulin M (IgM) antibodies hold promise as anticancer drugs and as agents for promoting immune homeostasis. This promise has not been realized due to low expression levels in mammalian cells producing IgM class antibodies, and the failure of protein A chromatography for IgM purification. Here, we describe a nonchromatographic platform for quantitatively capturing IgMs at neutral pH, which is then recovered with 86%-94% yield and >95% purity at pH 3.
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