Ten DNA markers were localized in the human genome by a screening procedure against the radiation hybrid somatic cell panel (GeneBridge 4 RH Panel) using polymerase chain reaction (RH mapping method). DNA markers were developed to nucleotide sequences adjacent to NotI sites of human chromosome 3 (NotI-STS markers) and also to nucleotide sequences of human cDNA (EST markers). Three EST markers mapped (B10164, S16R and 18F5R) were localized in the human genome for the first time. Marker B10164 was found to be homologous to the nucleotide sequence of the BASP1 gene coding a major receptor protein. Markers S16R and 18F5R presumably tagged new genes, because no homologies were revealed among the nucleotide sequences presented in the databases. For four NotI-STS, more precise localization on human chromosome 3 was determined. On the basis of the data obtained, the NotI map may be integrated with other types of physical maps of human chromosome 3. RH mapping with a standard commercial panel of radiation hybrid somatic cells provided a chance to integrate the data obtained into international databases and existing integrated human chromosomal maps.

Download full-text PDF

Source

Publication Analysis

Top Keywords

human genome
12
nucleotide sequences
12
human chromosome
12
human
8
panel radiation
8
dna markers
8
localized human
8
radiation hybrid
8
hybrid somatic
8
est markers
8

Similar Publications

Single-cell and spatial transcriptomics illuminate bat immunity and barrier tissue evolution.

Mol Biol Evol

January 2025

Shmunis School of Biomedicine and Cancer Research, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

Bats have adapted to pathogens through diverse mechanisms, including increased resistance - rapid pathogen elimination, and tolerance - limiting tissue damage following infection. In the Egyptian fruit bat (an important model in comparative immunology) several mechanisms conferring disease tolerance were discovered, but mechanisms underpinning resistance remain poorly understood. Previous studies on other species suggested that elevated basal expression of innate immune genes may lead to increased resistance to infection.

View Article and Find Full Text PDF

Long-read sequencing has emerged as a transformative technology in recent years, offering significant potential for the molecular diagnosis of unresolved genetic disorders. Despite its promise, the comprehensive detection and clinical annotation of genomic variants remain intricate and technically demanding. We present SUMMER, an integrated and structured workflow specifically designed to process raw Nanopore sequencing reads.

View Article and Find Full Text PDF

Reclassification of Salisediminibacterium haloalkalitolerans Sultanpuram et al. 2015 as a Later Heterotypic Synonym of Salisediminibacterium halotolerans Jiang et al. 2012.

Curr Microbiol

January 2025

Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou & School of Basic Medical Science & Institution of One Health Research, Guizhou Medical University, Guiyang, 561113, People's Republic of China.

In the present study, the taxonomic position of Salisediminibacterium haloalkalitolerans was evaluated by determining the 16S rRNA gene sequence similarity, genome relatedness, and phylogenetic analyses. The 16S rRNA gene sequences extracted from the genomes of Salisediminibacterium haloalkalitolerans 10nlg and Salisediminibacterium halotolerans DSM 26530 showed 100% similarity, supporting their classification as the same species. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between S.

View Article and Find Full Text PDF

Human metapneumovirus (HMPV) is an important causative agent of respiratory tract disease. Fundamental knowledge of the interaction between HMPV and the innate immune system could lead to the design of novel antiviral therapies. Previously, we demonstrated that HMPV M2-2 deletion mutants had hypermutated genomes and contained defective interfering particles (DIs), which are potent inducers of the IFN response.

View Article and Find Full Text PDF

LIMA1 Is a Prognostic Senescence-Inhibitory Gene in Head and Neck Squamous Carcinoma.

Oral Dis

January 2025

Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

Background: This study aimed to investigate potential cellular senescence inhibitory genes (CSIGs) and discover novel therapeutic targets in head and neck squamous cell carcinoma.

Methods: Dysregulated CSIGs were identified based on The Cancer Genome Atlas (TCGA) and the Human Aging Genomic Resources (HAGR) database. Prognostic value and immune infiltration were assessed through bioinformatic analysis.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!