We have investigated the molecular motions of polysaccharides of bovine nasal and pig articular cartilage by measuring the 13C NMR relaxation times (T1 and T2). Both types of cartilage differ significantly towards their collagen/glycosaminoglycan ratio, leading to different NMR spectra. As chondroitin sulfate is the main constituent of cartilage, aqueous solutions of related poly- and monosaccharides (N-acetylglucosamine and glucuronic acid) were also investigated. Although there are only slight differences in T1 relaxation of the mono- and the polysaccharides, T2 decreases about one order of magnitude, when glucuronic acid or N-acetylglucosamine and chondroitin sulfate are compared. It is concluded that the ring carbons are motion-restricted primarily by the embedment in the rigid pyranose structure and, thus, additional limitations of mobility do not more show a major effect. Significant differences were observed between bovine nasal and pig articular cartilage, resulting in a considerable line-broadening and a lower signal to noise ratio in the spectra of pig articular cartilage. This is most likely caused by the higher collagen content of articular cartilage in comparison to the polysaccharide-rich bovine nasal cartilage.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0008-6215(00)00064-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Curcumin and Vitamin D Supplement Attenuates Knee Osteoarthritis Progression in ACLT + MMx Rat Model: Effect on Cartilage Protection and Pain Reduction.
Nutrients
January 2025
Department of Anesthesiology, Cathay General Hospital, Taipei 280, Taiwan.
Knee osteoarthritis (OA) is a common and debilitating disorder marked by joint degradation, inflammation, and persistent pain. This study examined the possible therapeutic effects of curcumin and vitamin D on OA progression and pain in a rat knee OA model by anterior cruciate ligament transection and meniscectomy (ACLT + MMx). Male Wistar rats were categorized into five groups: control, curcumin-treated (100 mg/kg/day), vitamin D-treated (25 µg/kg/day), a combination of vitamin D and curcumin, and sham-operated.
View Article and Find Full Text PDFEffect of Open-Wedge High Tibial Osteotomy and Lateral Retinacular Release on the Articular Cartilage of the Patellofemoral Joint: Analysis Using Magnetic Resonance Imaging T2 Mapping.
J Clin Med
January 2025
Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
After open-wedge high tibial osteotomy (OWHTO), the patella is displaced distally, causing patellofemoral joint degeneration. The objective of this study was to ascertain whether the combination of OWHTO and lateral retinacular release (LRR) can prevent articular cartilage degeneration of the patellofemoral joint using magnetic resonance imaging T2 mapping. This study included 37 patients (37 knees) who underwent OWHTO alone (OWHTO group) and 37 patients (37 knees) who underwent OWHTO with LRR (OWHTO + LRR group) with a correction angle of <10° for varus knee osteoarthritis.
View Article and Find Full Text PDFPorcine-Derived Chondroitin Sulfate Sodium Alleviates Osteoarthritis in HTB-94 Cells and MIA-Induced SD Rat Models.
Int J Mol Sci
January 2025
Beirne B. Carter Center for Immunology Research, University of Virginia, Charlottesville, VA 22903, USA.
Osteoarthritis (OA) is a chronic disease characterized by cartilage degradation, leading to bone friction, inflammation, stiffness, pain, and reduced mobility. This study investigates the therapeutic effects of porcine-derived chondroitin sulfate sodium (CS) on OA symptoms at both cellular and animal levels. study, HTB-94 chondrocytes were treated with inflammatory stimuli and CS (10, 50, 100, and 200 μg/mL) to assess the release of inflammatory mediators and the expression of genes and proteins related to cartilage synthesis and degradation.
View Article and Find Full Text PDFEmerging Strategies in Cartilage Repair and Joint Preservation.
Medicina (Kaunas)
December 2024
Faculty of Medicine, Victor Babes University of Medicine and Pharmacy, 2 Eftimie Murgu, 300041 Timisoara, Romania.
Cartilage repair remains a critical challenge in orthopaedic medicine due to the tissue's limited self-healing ability, contributing to degenerative joint conditions such as osteoarthritis (OA). In response, regenerative medicine has developed advanced therapeutic strategies, including cell-based therapies, gene editing, and bioengineered scaffolds, to promote cartilage regeneration and restore joint function. This narrative review aims to explore the latest developments in cartilage repair techniques, focusing on mesenchymal stem cell (MSC) therapy, gene-based interventions, and biomaterial innovations.
View Article and Find Full Text PDFA meta-analysis of randomized controlled trials: evaluating the efficacy of isokinetic muscle strengthening training in improving knee osteoarthritis outcomes.
J Orthop Surg Res
January 2025
Medical College, Tibet University, Lhasa, Tibet, 850000, China.
Background: Knee osteoarthritis (KOA) is a prevalent degenerative joint disease. The primary pathological manifestations of KOA include articular cartilage degeneration, joint space narrowing, and osteophyte formation, leading to a spectrum of symptoms, including joint pain, stiffness, reduced mobility, diminished muscle strength, and severe disability. We aimed to utilize a meta-analysis to evaluate the efficacy of isokinetic muscle strengthening training (IMST) as a rehabilitation treatment for KOA in lowland areas.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!