Determination of branched-chain L-amino-acid aminotransferase activity.

Methods Enzymol

Deutsches Diabetes Forschungsinstitut, Klinische Biochemie, Düsseldorf, Germany.

Published: February 2001

Download full-text PDF

Source
http://dx.doi.org/10.1016/s0076-6879(00)24215-8DOI Listing

Publication Analysis

Top Keywords

determination branched-chain
4
branched-chain l-amino-acid
4
l-amino-acid aminotransferase
4
aminotransferase activity
4
determination
1
l-amino-acid
1
aminotransferase
1
activity
1

Similar Publications

Background: Branched-chain amino acids (BCAAs) are widely used as sports nutrition supplements. However, their impact on the rate of force development (RFD), an indicator of explosive muscle strength, has not yet been validated. This study aimed to assess the impact of BCAA supplementation on the RFD in college basketball players during simulated games.

View Article and Find Full Text PDF

Objective: This study investigates the effects of caloric restriction (CR) on renal injury and fibrosis following ischemia-reperfusion injury (IRI), with a focus on the roles of the mechanistic/mammalian target of rapamycin complex 1 (mTORC1) signaling and autophagy.

Methods: A mouse model of unilateral IRI with or without CR was used. Renal function was assessed through serum creatinine and blood urea nitrogen levels, while histological analysis and molecular assays evaluated tubular injury, fibrosis, mTORC1 signaling, and autophagy activation.

View Article and Find Full Text PDF

Background: Sarcopenia, a key aspect of malnutrition in liver cirrhosis (LC), affects 30-70% of LC patients. Given the inconsistent results from RCTs on branched-chain amino acids (BCAAs) for treating sarcopenia in LC, we conducted a systematic review and meta-analysis to assess the efficacy and safety of BCAAs for sarcopenia management in LC patients.

Methods: A systematic review and meta-analysis synthesizing evidence from RCTs obtained from PubMed, Embase, Cochrane, Scopus, and Web of Science from inception to April 2024.

View Article and Find Full Text PDF

In this study, the interactions between three quaternary ammonium salt (QAS) cationic surfactants with different branched-chain lengths (TMBAC, TEBAC, and TBBAC) and DNA are investigated by UV-vis absorption, fluorescence and CD spectroscopy, viscosity method, and gel electrophoresis. Berberine hydrochloride (BR) is utilized as a fluorescent probe. The three interaction modes and strengths are compared.

View Article and Find Full Text PDF

Four aliphatic amino acids-α-aminobutyric acid (AABA), β-aminobutyric acid (BABA), α-aminoisobutyric acid (AAIBA) and β-aminoisobutyric acid (BAIBA) were investigated in water as a solvent by two quantum chemical methods. B3LYP hybrid version of DFT was used for geometry optimization and a full vibrational analysis of neutral molecules, their cations and anions in the canonical and zwitterionic forms (6 forms for each species). Ab initio DLPNO-CCSD(T) method was applied in the geometry pre-optimized by B3LYP.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!