The article summarizes the biochemical researches carried out at Kharkiv Imperial University from the middle of XIX century up to the cessation of its existence in 1920 as a result of transformation into the Kharkiv Institute of National Education. Scientific activity at the Chair of Medical Chemistry at Medical Department is described in details. Information on professors who led the chair and their researches are represented. Among them a great attention is spared to the Kharkiv works of such famous scientists as A. Danilevsky and V. Gulevich, who made a great contribution to the development of Russian and world biochemistry. There are also some resordes about researches of biological and physiological chemistry carried out at other chairs of Medical Department and Department of Physics and Mathematics of the Kharkiv University. In particular, the article presents the works of well-known plant physiologists and biochemists prof. V. Palladin and V. Zalessky, and the endocrinological researches led by prof. A. Reprev.

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