We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92266 | PMC |
| http://dx.doi.org/10.1128/AEM.66.9.4112-4114.2000 | DOI Listing |
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