Early papers revealed that culture filtrate of Byssochlamys nivea has antibiotic properties. Active filtrate of B. nivea is extracted with ethyl acetate. Paper chromatography is carried out on Whatman no. 1; thin-layer chromatography is carried out on Kieselgel; visualization with bioautographic technique reveals a single spot. Ethyl acetate extract is passed through silicic acid-celite column. Each isolated fraction is tested for antibiotic activity. Only one supports this activity; a crystallized matter, melting point 109-110 degrees C, thermostable, is obtained.
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