Severity: Warning
Message: fopen(/var/lib/php/sessions/ci_session5fh30nnhobkhvdndkmpm7nd70su9s5ur): Failed to open stream: No space left on device
Filename: drivers/Session_files_driver.php
Line Number: 177
Backtrace:
File: /var/www/html/index.php
Line: 316
Function: require_once
Severity: Warning
Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)
Filename: Session/Session.php
Line Number: 137
Backtrace:
File: /var/www/html/index.php
Line: 316
Function: require_once
Human hepatocytes (HH) are an optimal in vitro model for the study of xenobiotics metabolism and toxicity. However, there is still a problem of HH availability. A promising possibility for the storage of HH for later utilization is cryopreservation. Procedures and conditions used for cryopreservation of human hepatocytes and hepatocytes of other species are presented in the present paper. Compared are some biochemical parameters characterizing viability and functionality of fresh and thawed HH.
Download full-text PDF |
Source |
---|
Acta Biomater
March 2025
Department of Biomedical Engineering, University of Illinois Chicago, Chicago, Illinois 60607, USA. Electronic address:
In vitro human liver models are indispensable for compound metabolism/toxicity screening, disease modeling, and regenerative medicine. While induced pluripotent stem cell-derived human hepatocyte-like cells (iHeps) mitigate the sourcing limitations with primary human hepatocytes (PHHs), their functional maturity is rate-limiting for application use. During development, immature hepatoblasts interact with different non-parenchymal cell (NPC) types, such as mesenchyme and endothelia, in a spatiotemporal manner to progress through functional maturation.
View Article and Find Full Text PDFChemosphere
March 2025
Instituto Universitario Mixto de Tecnología Química (UPV-CSIC), Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, 46022-Valencia, Spain. Electronic address:
This work was undertaken to address the potential environmental impact of the UVA filter Uvinul® A Plus (DHHB) upon its biotransformation in humans. For this purpose, the putative human metabolite 3 was prepared by a three-step synthetic sequence involving the initial Koenigs-Knorr reaction of 2-(4-diethylamino-2-hydroxybenzoyl)benzoic acid (DHB) with acetobromo-α-d-glucuronic acid methyl ester, which afforded the corresponding peracetylated DHB-acyl glucuronide (1). Subsequent enzymatic deprotection with amano lipase A (LAS) led to the 2-(4-diethylamino-2-hydroxybenzoyl)benzoyl-β-D-glucuronide methyl ester (2).
View Article and Find Full Text PDFMol Biol Rep
March 2025
Department of Infectious Disease, The Third Hospital of Hebei Medical University, No. 68 Xiangjiang Road, Shijiazhuang, 050051, China.
Background: The roles of microRNAs in the regulation of autophagy and apoptosis in hepatic cells suggest that they may serve as novel biomarkers or therapeutic targets for various liver injuries. In this study, we aim to analyze whether miR-124a regulates autophagy and apoptosis in hepatic cells, particularly in acute-on-chronic liver failure (ACLF).
Materials And Methods: The plasma and liver tissues from the healthy control (HC) and ACLF patients were included.
Genome Biol
March 2025
Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA.
Background: The molecular underpinnings of organ dysfunction in severe COVID-19 and its potential long-term sequelae are under intense investigation. To shed light on these in the context of liver function, we perform single-nucleus RNA-seq and spatial transcriptomic profiling of livers from 17 COVID-19 decedents.
Results: We identify hepatocytes positive for SARS-CoV-2 RNA with an expression phenotype resembling infected lung epithelial cells, and a central role in a pro-fibrotic TGFβ signaling cell-cell communications network.
ACS Appl Mater Interfaces
March 2025
Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
Mesenchymal stem cells (MSCs) play a crucial role in regenerative medicine due to their regenerative potential. However, traditional MSC-based therapies are hindered by issues such as microvascular obstruction and low cell survival after transplantation. Exosomes derived from MSCs (MSC-Exo) provide a cell-free, nanoscale alternative, mitigating these risks and offering therapeutic potential for liver diseases.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!