Among two pairs of agnoproteins encoded in upstream positions in the late mRNAs of avian polyomavirus BFDV, either agno-1a or its splice derivative agno-1b are required for viral propagation. Out of the two proteins both of which consist of multiple electrophoretic subspecies, the smaller and less complex agno-1b has been cDNA-cloned into an influenza-virus /RNA-polymerase I expression system for production of higher amounts of this protein in infected chicken embryo fibroblasts. Fractional modification of agno-1b by phosphorylation at residues serine 51, serine 53, and threonine 73 is demonstrated through dephosphorylation by alkaline phosphatase, mass spectrometry of individual protein species isolated by strong anion exchange chromatography, and single or multiple alanine substitutions of serine or threonine residues in site-directed mutagenesis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s007050070120 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel polyomavirus in the endangered garden dormouse Eliomys quercinus.
Virol J
November 2024
Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio al. 7, Vilnius, LT-10257, Lithuania.
Article Synopsis
- The garden dormouse's population in Europe has declined significantly due to factors like habitat loss, climate change, and potentially pathogen exposure.
- In a study involving 89 garden dormice from Germany, researchers tested kidney samples for polyomavirus DNA and checked for antibodies in their body fluids.
- A new polyomavirus related to other known polyomaviruses was identified, with a small percentage of dormice testing positive for the virus, suggesting that further research is needed to determine its significance and specificity to the garden dormouse.
Detection and molecular characterization of avian polyomavirus in budgerigar and non-budgerigar psittacine species in bird markets of Pakistan.
Comp Immunol Microbiol Infect Dis
December 2024
Department of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
Article Synopsis
- * A study investigated the presence of these viruses in feather and tissue samples, finding a 12% positivity rate for Avian Polyomavirus in Psittacine birds, with particular emphasis on budgerigars.
- * The research highlights the lack of data on Avian Polyomavirus in Pakistan and serves as a foundational step for future studies on diagnosis and vaccine development.
Variation in Plumage Coloration of Rosy-Faced Lovebirds (Agapornis roseicollis): Links to Sex, Age, Nutritional Condition, Viral Infection, and Habitat Urbanization.
J Exp Zool A Ecol Integr Physiol
January 2025
School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
Expression of vibrant plumage color plays important communication roles in many avian clades, ranging from penguins to passerines, but comparatively less is known about color signals in parrots (order Psittaciformes). We measured variation in coloration from three plumage patches (red face, blue rump, red tail) in an introduced population of rosy-faced lovebirds (Agapornis roseicollis) in Phoenix, Arizona, USA and examined color differences between the sexes and ages as well as relationships with several indices of quality, including disease presence/absence (infection with beak and feather disease, Circovirus parrot, and a polyomavirus, Gammapolyomavirus avis), nutritional state (e.g.
View Article and Find Full Text PDFAvian Polyomavirus Among Psittacine Birds in Iran: Molecular Detection Rate and Associated Risk Factors.
J Avian Med Surg
April 2024
Biotechnology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Aftab 24, Amol, Iran.
Avian polyomavirus (APV) infection causes various health problems in psittacine species, including death. The present study was conducted to investigate the prevalence of APV among psittacine birds in Iran. We also aimed to evaluate the impact of age, sex, species, season, and origin of the birds on the prevalence of APV.
View Article and Find Full Text PDFPrevalence, genotypes, and infection risk factors of psittacine beak and feather disease virus and budgerigar fledgling disease virus in captive birds in Hong Kong.
Arch Virol
April 2024
School of Biological Sciences, The University of Hong Kong, Pok Fu Lam Road, Hong Kong, China.
Article Synopsis
- Psittacine beak and feather disease virus (PBFDV) and budgerigar fledgling disease virus (BFDV) are harmful to birds, especially parrots, and were tested in a study involving 516 birds in Hong Kong.
- The study found PBFDV in 7.17% of samples and BFDV in 0.58%, with higher PBFDV rates noted in pet shops and some PBFDV cases in non-parrot species.
- Genetic analysis showed the Hong Kong PBFDV strains were closely related to those in Europe and Asia, prompting recommendations for ongoing virus monitoring and minimizing contact between captive and wild birds.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!