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Efficient Simulation of Viral Transduction and Propagation for Biomanufacturing.
ACS Synth Biol
October 2024
Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
The design of biomanufacturing platforms based on viral transduction and/or propagation poses significant challenges at the intersection between synthetic biology and process engineering. This paper introduces vitraPro, a software toolkit composed of a multiscale model and an efficient numeric technique that can be leveraged for determining genetic and process designs that optimize transduction-based biomanufacturing platforms and viral amplification processes. Viral infection and propagation for up to two viruses simultaneously can be simulated through the model, considering viruses in either the lytic or lysogenic stage, during batch, perfusion, or continuous operation.
View Article and Find Full Text PDFVirus-like Particles vaccine based on co-expression of G5 Porcine rotavirus VP2-VP6-VP7 induces a powerful immune protective response in mice.
Vet Microbiol
November 2024
Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science. Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu 610064, China. Electronic address:
Article Synopsis
- Porcine rotavirus (PoRV) is a major cause of severe diarrhea in young piglets, leading to high morbidity and mortality in the pig farming industry, with the G5 genotype being the most prevalent strain.
- Researchers developed a vaccine using an insect cell-baculovirus system that produced virus-like particles (VLPs) containing key rotavirus proteins (VP2, VP6, and VP7), resembling the natural rotavirus structure.
- Immunization trials in mice showed the VLPs effectively triggered strong immune responses and reduced PoRV levels, indicating their potential as an effective vaccine for preventing Porcine Rotavirus infections.
Establishment of human post-vaccination SARS-CoV-2 standard reference sera.
J Immunol Methods
July 2024
Iowa City Veterans Administration Healthcare System, 601 Highway 6, Iowa City, IA 52246, USA; The University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA. Electronic address:
There is a critical need to understand the effectiveness of serum elicited by different SARS-CoV-2 vaccines against SARS-CoV-2 variants. We describe the generation of reference reagents comprised of post-vaccination sera from recipients of different primary vaccines with or without different vaccine booster regimens in order to allow standardized characterization of SARS-CoV-2 neutralization in vitro. We prepared and pooled serum obtained from donors who received a either primary vaccine series alone, or a vaccination strategy that included primary and boosted immunization using available SARS-CoV-2 mRNA vaccines (BNT162b2, Pfizer and mRNA-1273, Moderna), replication-incompetent adenovirus type 26 vaccine (Ad26.
View Article and Find Full Text PDFComparing ATPase activity of ATP-binding cassette subfamily C member 4, lamprey CFTR, and human CFTR using an antimony-phosphomolybdate assay.
Front Pharmacol
February 2024
Division of Pulmonology, Asthma, Cystic Fibrosis, and Sleep, Department of Pediatrics, Emory + Children's Center for Cystic Fibrosis and Airways Disease Research, Emory University School of Medicine and Children's Healthcare of Atlanta, Atlanta, GA, United States.
ATP-binding cassette (ABC) transporters use the hydrolysis of ATP to power the active transport of molecules, but paradoxically the cystic fibrosis transmembrane regulator (CFTR, ABCC7) forms an ion channel. We previously showed that ATP-binding cassette subfamily C member 4 (ABCC4) is the closest mammalian paralog to CFTR, compared to other ABC transporters. In addition, Lamprey CFTR (Lp-CFTR) is the oldest known CFTR ortholog and has unique structural and functional features compared to human CFTR (hCFTR).
View Article and Find Full Text PDFEffect of mechanical stresses on viral capsid disruption during droplet formation and drying.
Colloids Surf B Biointerfaces
January 2024
Department of Chemical and Biological Engineering, University of Colorado Boulder, CO 80303, United States. Electronic address:
Identification of the mechanisms by which viruses lose activity during droplet formation and drying is of great importance to understanding the spread of infectious diseases by virus-containing respiratory droplets and to developing thermally stable spray dried live or inactivated viral vaccines. In this study, we exposed suspensions of baculovirus, an enveloped virus, to isolated mechanical stresses similar to those experienced during respiratory droplet formation and spray drying: fluid shear forces, osmotic pressure forces, and surface tension forces at interfaces. DNA released from mechanically stressed virions was measured by SYBR Gold staining to quantify viral capsid disruption.
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