Activation of nuclear factor kappaB (NF-kappaB) is thought to be required for cytokine production by lipopolysaccharide (LPS)-responsive cells. Here, we investigated the contribution of NF-kappaB in preventing LPS-induced transcription of the tumor necrosis factor alpha (TNF-alpha) gene in a murine macrophage cell line, P388D1, when tolerance was induced in the cells with a short exposure to a higher dose of LPS. Electrophoretic mobility shift assays with the kappaB elements of the murine TNF-alpha promoter and enhancer revealed that nuclear mobilization of heterodimers of p65/p50, c-rel/p50 and p65/c-rel, and homodimers of p65 was markedly reduced in LPS-tolerant cells, whereas that of p50 homodimers was only slightly increased. Western blot analysis showed that the phosphorylation of Ser32 on IkappaBalpha and its transient degradation did not occur in LPS-tolerant cells. These results thus suggest that desensitization of TNF-alpha gene expression in this LPS-tolerant state is closely associated with down-regulation of transactivating NF-kappaB and may involve a defect in the LPS-induced IkappaBalpha kinase pathway.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Infusion Reactions to Infliximab in Pediatric Patients with Inflammatory Bowel Disease.
Children (Basel)
November 2024
Division of Gastroenterology, Department of Pediatrics, University of New Mexico, Albuquerque, NM 87131, USA.
Infliximab (IFX) is a recombinant DNA-derived chimeric IgG monoclonal antibody protein that inhibits tumor necrosis factor alpha (TNF-α). IFX, like other agents derived from foreign proteins, can cause infusion reactions both during and after the infusion. The incidence of infusion reactions ranges between 0% and 15% in pediatric patients.
View Article and Find Full Text PDFEarly Sacral Neuromodulation Prevented Detrusor Overactivity in Rats With Spinal Cord Injury.
Neuromodulation
July 2024
Department of Urology, Qilu Hospital of Shandong University, Jinan, China; Key Laboratory of Urinary Precision Diagnosis and Treatment in Universities of Shandong, Jinan, China. Electronic address:
Article Synopsis
- The study explores the effect of sacral neuromodulation (SNM) on bladder dysfunction in rats with spinal cord injury, focusing on its mechanism during the bladder-areflexia phase.
- It uses various methods like physiological measurements, staining techniques, and urodynamic tests to assess SNM's impact on bladder health and inflammation.
- Results indicate that early SNM enhances bladder capacity and efficiency while preventing urothelial edema, suggesting potential therapeutic benefits for neurogenic bladder dysfunction without major adverse effects.
Purified Free Mannan Promotes Tolerogenic Responses in Peanut-Stimulated Human Dendritic Cells.
Int Arch Allergy Immunol
July 2024
Department of Biochemistry and Molecular Biology, School of Chemistry, Complutense University, Madrid, Spain.
Introduction: IgE-mediated peanut allergy is an important public health problem of increasing prevalence leading to anaphylactic reactions both in children and adults. Allergen-specific oral immunotherapy (OIT) is the single treatment with the potential capacity to modify the course of the disease, but it still faces some drawbacks in terms of efficacy, safety, patients' adherence, and cost. Alternative strategies, including the use of novel adjuvants, to overcome such limitations are highly demanded.
View Article and Find Full Text PDFCannabidiol Alleviates Chronic Prostatitis and Chronic Pelvic Pain Syndrome CB2 Receptor Activation and TRPV1 Desensitization.
World J Mens Health
January 2025
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Article Synopsis
- The study investigates how cannabidiol (CBD) affects inflammation caused by lipopolysaccharide (LPS) and the chronic pelvic pain syndrome (CP/CPPS) using both human cell models and rat subjects.
- * In lab tests, CBD showed a significant reduction in inflammatory markers and pain-related receptors, suggesting it mitigates inflammation through specific biological pathways.
- * The research concludes that CBD could be an effective therapy for managing CP/CPPS due to its ability to lower pain and inflammation.
TH1/TH2 repolarization in induction of immune tolerance to non-steroidal anti-inflammatory drugs during the management of sickle cell disease vaso-occlusive crisis.
Egypt J Immunol
October 2023
Félix Houphouet Boigny University-Medical sciences-Immunology Allergology Department, Université of Cocody, Abidjan, Côte d'Ivoire.
Respiratory manifestations related to the intake of non-steroidal anti-inflammatory drugs (NSAIDs) during the treatment of the painful vaso-occlusive crisis of sickle cell disease are either a type I hypersensitivity mechanism of the Gell and Coombs classification, or a pharmacological mechanism of NSAIDs. The use of NSAIDs is essential in the Abidjan school because of the absence of therapeutic alternatives in the management of the inflammatory crisis of this disease. The induction of tolerance to NSAIDs initiated by the authors has had clear clinical success.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!