Nickel-plated slides were prepared by evaporating a nickel layer (congruent to 4000 A thick) on glass slides in the presence of a magnetic field whose lines of force were perpendicular to the surface of the slides. Such slides are called active. After being coated with a layer of bovine albumin, they could absorb a layer of antibodies 70-80 A thick. However, if the active slides before they were coated with bovine serum albumin, were submitted to a magnetic field with lines of force parallel to the surface, the layer of antibodies absorbed was only 40 A thick. They had become inactive. It has been found that slides remain active at night but that shortly after sunrise they become slowly inactivated and reach a minimum in their activity at exactly the midday period. They regain full activity at sunset. It is shown that the inactivation results from a solar radiation that can be stopped by 3.5 cm of lead. On December 13th, 1974 there was an eclipse of the sun with 65% occultation at noon (Daylight Saving Time). The activity of the slide at noon was 65% of the maximum activity (83 A) observed before sunrise. The thickness of the adsorbed layer of antibodies were 75 A instead of 63 A observed in the absence of the eclipse. The activation of the slides originates in a radiation of non-solar origin that is adsorbed by 1 can of lead.
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http://dx.doi.org/10.1073/pnas.72.6.2462 | DOI Listing |
J Vasc Interv Radiol
January 2025
Department of Diagnostic and Interventional Radiology, Osaka University Graduate School of Medicine. 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Purpose: This research aimed to develop and assess a Lipiodol Pickering emulsion containing anti-Programmed cell Death Ligand 1 (PD-L1) antibodies through in vitro experiments.
Materials And Methods: The emulsion was created by combining Lipiodol with poly (lactic-co-glycolic acid) (PLGA) nanoparticles and anti-PD-L1 antibodies. Confocal laser microscopy was used to evaluate the encapsulation of the antibodies within the Pickering emulsion.
Cell
January 2025
University of Chinese Academy of Sciences, Beijing, China; Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address:
The cerebral cortex and hippocampus are crucial brain regions for learning and memory, which depend on activity-induced synaptic plasticity involving N-methyl-ᴅ-aspartate receptors (NMDARs). However, subunit assembly and molecular architecture of endogenous NMDARs (eNMDARs) in the brain remain elusive. Using conformation- and subunit-dependent antibodies, we purified eNMDARs from adult rat cerebral cortex and hippocampus.
View Article and Find Full Text PDFSmall Methods
January 2025
Laboratory of Analytical Chemistry, Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, via Monteroni, Lecce, 73100, Italy.
Molecularly Imprinted Polymers (MIPs) have gained prominence as synthetic receptors, combining simplicity of synthesis with robust molecular recognition akin to antibodies and enzymes. One of their main application areas is chemical sensing. However, direct integration of MIPs with nanostructured transducers, crucial for enhancing sensing capabilities and broadening MIPs sensing applications, remains limited.
View Article and Find Full Text PDFCNS Neurosci Ther
January 2025
Department of Neurology, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: Multiple sclerosis (MS) is an autoimmune disorder affecting the central nervous system, with varying clinical manifestations such as optic neuritis, sensory disturbances, and brainstem syndromes. Disease progression is monitored through methods like MRI scans, disability scales, and optical coherence tomography (OCT), which can detect retinal thinning, even in the absence of optic neuritis. MS progression involves neurodegeneration, particularly trans-synaptic degeneration, which extends beyond the initial injury site.
View Article and Find Full Text PDFBiosensors (Basel)
January 2025
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210092, China.
Herein, a sensitive electrochemiluminescence (ECL) immunosensor is designed by immobilizing ruthenium-tagged immune complexes at flexible poly-ethylene-glycol (PEG) chains on the electrode surface, which offers more freedom for the collision of the ruthenium complex at the electrode during the initial ECL reaction. The electrochemical characterizations confirm the loose structure of the assembled layer with the immune complex, providing an increase in the current and the resultant enhanced ECL emissions. Comparing the sensors with the rigid structure, a 34-fold increase in the maximal ECL emission is recorded when PEG3400 is used as a linker.
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