A novel configuration of bioartificial liver support system based on circulating microcarrier culture.

Artif Cells Blood Substit Immobil Biotechnol

Institute for Molecular Biology, Nankai University, Tianjin, PR China.

Published: July 2000

The purpose of this investigation is to initiate a new bioartificial liver support system that utilizes circulating microcarrier cultures in the extracapillary space of a hollow fiber cartridge. The material exchange occurs on the membranes of the hollow fiber. Toxins are metabolized by the circulating cells on the microcarriers driven by a centrifugal pump. We inoculated 2-3 x 10E8 Hep G2 cells on 2.5 grams of Cytodex 3 microcarriers, and allowed them flowing in the extracapillary space of a modified plasma filter. 10% FCS Medium was pumped through the capillaries at different rates. Cells keep morphological integrity and functionality during the circulation. These preliminary results suggest that this configuration of a bioartificial liver support system offers a future investigation.

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Source
http://dx.doi.org/10.3109/10731190009119359DOI Listing

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