Silica spheres were prepared by the Stöber method, and the method of labeling with pyrene, using aminopropylysilane as the linking agent, was optimized. Excimers between particles were formed when high concentrations of label were used. Both surfactant and polyelectrolyte addition were able to cause floc formation when added to suspensions of the silica, but only the surfactant changed the I(1)/I(3) ratio and diminished excimer formation. Copyright 2000 Academic Press.
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http://dx.doi.org/10.1006/jcis.2000.6937 | DOI Listing |
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.
Accurate and sensitive fluorescence imaging of biothiols is essential for understanding the mechanism underlying some physiological and pathological events, as well as the prevention and diagnosis of diseases. However, low signal transduction efficiency and poor biocompatibility of fluorescence tags associated with current sensors hinder their potential utilizations. Herein, a smart biothiols sensitive vivo imaging platform on the basis of amplifying nanoprobe has been designed.
View Article and Find Full Text PDFJ Phys Chem B
February 2023
Institute for Polymer Research, Waterloo Institute for Nanotechnology, Department of Chemistry, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
The polypeptide PGlyAlaGlu was prepared with 20 mol % glycine (Gly), 36 mol % d,l-alanine (Ala), and 44 mol % d,l-glutamic acid (Glu) and labeled with the dye 1-pyrenemethylamine to yield a series of Py-PGlyAlaGlu samples. The fluorescence decays of the Py-PGlyAlaGlu samples were analyzed according to the fluorescence blob model (FBM) to obtain the number of amino acids (aa's) encompassed inside the subvolume of the polypeptide probed by an excited pyrene. An value of 29 (±2) was retrieved for Py-PGlyAlaGlu, which was much larger than for any of the copolypeptide PGlyGlu or PAlaGlu prepared with either Gly and Glu or Ala and Glu, respectively.
View Article and Find Full Text PDFLangmuir
June 2022
Institute for Polymer Research, Waterloo Institute for Nanotechnology, Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
The gemini surfactant PyO-3-12, made of two dimethylammonium bromides joined by a propyl linker and bearing a dodecyl pendant on one side and a 1-pyrenemethoxyhexyl group on the other side, was employed to probe the interactions between positively charged PyO-3-12 and negatively charged sodium dodecyl sulfate (SDS). PyO-3-12 was selected for its ability to respond to the polarity of its local environment through the fluorescence intensity ratio / of the first-to-third fluorescence peaks of the pyrene monomer and the local pyrene concentration [] through the / ratio of the pyrene excimer-to-pyrene monomer fluorescence intensity. Furthermore, analysis of the fluorescence decays of aqueous solutions of PyO-3-12 and SDS yielded a measure of the internal dynamics, local concentration, and state (associated vs unassociated) of PyO-3-12 in solution.
View Article and Find Full Text PDFACS Omega
April 2022
Department of Applied Chemistry, School of Science, Anhui Agricultural University, Hefei 230036, China.
The extensive use of organophosphorus pesticides in agriculture poses a high risk to human health and has boosted the demands for developing sensitive monitoring methods. Herein, we developed a facile and sensitive method for isocarbophos detection based on the remarkable fluorescence enhancement of pyrene during host-guest interaction of β-cyclodextrin polymer (β-CDP) and pyrene. The 3'-pyrene-labeled isocarbophos aptamer could be cleaved by exonuclease I to obtain free pyrene that was tagged on mononucleotides, which could enter the hydrophobic cavity of β-CDP, resulting in a prominent fluorescence enhancement.
View Article and Find Full Text PDFLangmuir
November 2021
Institute for Polymer Research, Waterloo Institute for Nanotechnology, Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
The cationic gemini surfactant PyO-3-12 was designed to include two dimethyl ammonium groups, one dodecyl tail, and 1-pyrenemethyl hexyl ether tail into the structure of the surfactant. The pyrenyl label ensured that the fluorescence of pyrene could be employed to probe the behavior of PyO-3-12 at the molecular level. The introduction of the oxygen atom in the β-position to pyrene was found to be critical for restoring the sensitivity of the pyrenyl label to the polarity of its environment.
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