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Molecular Understanding of Activity Changes of Alcohol Dehydrogenase in Deep Eutectic Solvents.
J Phys Chem B
January 2025
Institute of Thermal Separation Processes, Hamburg University of Technology, Eißendorfer Straße 38, Hamburg 21073, Germany.
Deep eutectic solvents (DESs) have emerged as promising solvents for biocatalysis. While their impact on enzyme solvation and stabilization has been studied for several enzyme classes, their role in substrate binding is yet to be investigated. Herein, molecular dynamics (MD) simulations of horse-liver alcohol dehydrogenase (HLADH) are performed in choline chloride-ethylene glycol (ChCl-EG) and choline chloride-glycerol (ChCl-Gly) at varying water concentrations.
View Article and Find Full Text PDFMolecular cloning and heterologous expression of lipase gene from Pseudomonas aeruginosa in Escherichia coli.
Int J Biol Macromol
January 2025
College of Technology and Engineering, MPUAT, Udaipur, Rajasthan-313001, India. Electronic address:
Lipases, enzymes that perform the hydrolysis of triglycerides into fatty acids and glycerol, present a potential paradigm shift in the realms of food and detergent industries. Their enhanced efficiency, energy conservation and environmentally friendly attributes make them promising substitutes for chemical catalysts. Motivated by this prospect, this present study was targeted on the heterologous expression of a lipase gene, employing E.
View Article and Find Full Text PDFYam starch-based sustainable edible films loaded with bioactive components from aroeira leaf extract: Mechanical, physical, and antioxidant properties.
Int J Biol Macromol
January 2025
Graduate Program in Food Science and Technology, Federal University of Paraíba, 58051-900 João Pessoa, Paraíba, Brazil. Electronic address:
This study aimed to investigate the impact of adding aroeira leaf extract (Schinus terebinthifolius Raddi) to a yam starch film matrix, focusing on the development of potentially active films and the evaluation of their physicochemical, mechanical, optical, and antioxidant properties. Films were produced using the casting method with varying extract concentrations (0, 3, 6, 12, and 15 %), yam starch (2 %), and glycerol (1 %). The antioxidant properties were analyzed by determining the total phenolic content, 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging, ferric reducing power, and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical elimination, which revealed a significant increase in antioxidant properties as the extract concentration increased.
View Article and Find Full Text PDFBioinspired bicontinuous adhesive hydrogel for wearable strain sensor with high sensitivity and a wide working range.
J Colloid Interface Sci
January 2025
Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education, Nanjing University of Science and Technology, Nanjing 210094 China. Electronic address:
Conductive hydrogel strain sensors demonstrate extensive potential in artificial robotics, human-computer interaction, and health monitoring, owing to their excellent flexibility and biocompatibility. Wearable strain sensors for real-time monitoring of human activities require hydrogels with self-adhesion, desirable sensitivity, and wide working range. However, balancing the high sensitivity and a wide working range remains a challenge.
View Article and Find Full Text PDFInsights on the role of cryoprotectants in enhancing the properties of bioinks required for cryobioprinting of biological constructs.
J Mater Sci Mater Med
January 2025
Tissue Engineering & Additive Manufacturing (TEAM) Lab, Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), ABCDE Innovation Centre, School of Chemical & Biotechnology (SCBT), SASTRA Deemed University, Thanjavur, 613401, India.
Preservation and long-term storage of readily available cell-laden tissue-engineered products are major challenges in expanding their applications in healthcare. In recent years, there has been increasing interest in the development of off-the-shelf tissue-engineered products using the cryobioprinting approach. Here, bioinks are incorporated with cryoprotective agents (CPAs) to allow the fabrication of cryopreservable tissue constructs.
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