Using microfluorescence in combination with image-analysis techniques we monitored intracellular calcium ([Ca(2+)](i)) dynamics in mouse islets of Langerhans loaded with fura-2 and recorded in vivo. [Ca(2+)](i) oscillates in the glycaemias range 5-10 mM, the duration of the oscillations being directly proportional to the blood glucose concentration. The analysis of different areas within the same islet shows that [Ca(2+)](i) oscillations are synchronous throughout the islet. These results show that in vivo, individual islets of Langerhans behave as a functional syncytium and suggest the existence of secretory pulses of insulin.
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