AI Article Synopsis
- The study investigated the role of the VP6 protein in enhancing the production of neutralizing antibodies (NeuAb) against rotavirus proteins VP7 and VP4 in BALB/c mice.
- Mice that were immunized with recombinant VP6 showed increased levels of serum NeuAb, specifically IgM and IgG, and cleared an oral rotavirus infection faster than the control group.
- The findings suggest that VP6-specific T-helper cells can assist B cells targeting VP7 and VP4, indicating a potential heterotypic response during rotavirus infection.
Article Abstract
We studied in the rotavirus system whether the internal protein VP6 could prime for an enhancement in the production of neutralizing antibodies (NeuAb) against the external proteins, VP7 and VP4. It was found that BALB/c mice immunized with recombinant YM VP6 protein, and challenged i.p. with a heterologous rotavirus had an increase in the production of serum NeuAb compared to the control. This response was both homotypic and heterotypic, and involved an increase of NeuAb belonging to the IgM and IgG isotypes. Mice immunized with the recombinant VP6 and challenged orally with infectious murine rotavirus cleared the infection one day earlier than the control not immunized with VP6, and showed a small increase of total fecal anti-rotavirus IgA. The results suggest that the T-helper (Th) cells specific for VP6 can provide cognate help to B cells specific for neutralizing epitopes on the VP7 and/or VP4 molecules, and that this help can be heterotypic. Finally, we provide evidence that this phenomenon may be happening during infection.
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| http://dx.doi.org/10.1007/s007050050674 | DOI Listing |
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