AI Article Synopsis
- The study examines methods for isolating fetal cells from maternal blood in women with male fetuses, utilizing optimized protocols.
- The fetal cells were detected using FISH techniques, showing varying percentages of fetal cell recovery: 2.1% through FACS, 3.8% via magnetic sorting, and 2.6% with two-stage density gradient precipitation.
- The findings suggest that this non-invasive method is promising for prenatal diagnosis of chromosomal conditions.
Article Abstract
This paper presents the results of investigation of the isolated from maternal blood by 4 different methods according to the optimized protocols. All women had male fetuses. The mononuclear cells with fetal erythroblasts were preisolated by using density-gradient centrifugation of the maternal blood in the Ficoll solution. Fetal cells were detected by FISH for Y-chromosomal sequences. The fetal cells were 2.1% by fluorescence activated cell sorting (FACS); 3.8% by magnetic field sorting, and 2.6% by two-stage density gradient precipitation. The fetal lymphocytes were investigated through air-cultivation of peripheral maternal lymphocytes. Their proportion was 3.9% in the culture samples. The findings lead to the conclusion that the new non-invasive approach is useful for prenatal diagnosis of chromosomal aneuploidies.
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