Ribosomal rRNA gene fragments (rDNA) encompassing part of the 16S rDNA, the 16S-23S rDNA spacer region and part of the 23S rDNA of 229 Neisseria gonorrhoeae strains were enzymatically amplified using conserved primers. The fragments of approximately 1200 bp were subjected to restriction analysis with HinfI. This revealed 13 patterns (patterns I-XIII) of which patterns I (78 strains), II (32 strains), III (38 strains) and IV (56 strains) were the most abundant, comprising 89.1% of the strains. The obtained restriction patterns consisted of 3 to 8 bands, ranging in size from 32 to 854 bp. The sum of the obtained bands was about 1200 bp for patterns I, II, III, IV, V, IX, and XIII. However, for patterns VI, VII, VIII, X, XI and XII, the sum of the bands well exceeded the estimated size of approximately 1200 bp. We demonstrated that this results from sequence divergence in the 4 rRNA operons, present in the genome of N. gonorrhoeae, giving rise to patterns that are a combination of several other patterns.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0723-2020(00)80040-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
New insights into freshwater ascomycetes: discovery of novel species in diverse aquatic habitats.
Front Cell Infect Microbiol
January 2025
Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China.
During investigations of freshwater fungi in Hunan and Yunnan provinces, China, sp. nov. (Nectriaceae), sp.
View Article and Find Full Text PDFClimatically Specialized Lineages of Batrachochytrium dendrobatidis, and its Likely Asian Origins.
Ecohealth
January 2025
Guangxi Key Laboratory for Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning, Guangxi, 530000, People's Republic of China.
Chytridiomycosis is a wildlife disease that has caused significant declines in amphibian populations and species extinctions worldwide. Asia, where the causal pathogens Batrachochytrium dendrobatidis (Bd) and B. salamndrivorans (Bsal) originated, has not witnessed mass die-offs.
View Article and Find Full Text PDFIsolation and Identification of Apiospora intestini from Hedera nepalensis Leaf Blight and Determination of Antagonism of Phomopsis sp.
Curr Microbiol
January 2025
College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming, 650224, China.
In order to identify the pathogen responsible for Hedera nepalensis leaf blight and investigate effective biocontrol strategies, samples were collected from 10 significantly infected areas at Southwest Forestry University; four to six infected leaves were gathered from each area, followed by the isolation and purification of strains from the infected plant leaves using tissue isolation and hyphae-purification techniques. We conducted an examination of the biological characteristics and compared the inhibitory effects of different concentrations of Phomopsis sp. (50%, 25%, 16.
View Article and Find Full Text PDFA Case of Subcutaneous Phaeohyphomycosis Caused by Exophiala xenobiotica in a Poorly-Controlled Diabetic Patient: The Conventional Papanicolaou Staining on Cytology Specimen Can Potentially Guide Us to the Correct Diagnosis.
Diagn Cytopathol
January 2025
Department of Pathology, Kanazawa Medical University Hospital, Ishikawa, Japan.
Background: Phaeohyphomycosis is a very rare fungal infection, which is one of more usual complications in immunocompromised and/or traumatic patients, has never been reported especially in a cytological field. We describe a first case of subcutaneous phaeohyphomycosis caused by Exophiala xenobiotica (E. xenobiotica) in a poorly controlled diabetic patient, and in which a correct cytological diagnosis of phaeohyphomycosis was possible to conclude.
View Article and Find Full Text PDFImage-Based Detection and Classification of Malaria Parasites and Leukocytes with Quality Assessment of Romanowsky-Stained Blood Smears.
Sensors (Basel)
January 2025
Space Robotics Research Group (SpaceR), Interdisciplinary Centre for Security, Reliability and Trust (SnT), University of Luxembourg, L-1855 Luxembourg, Luxembourg.
Malaria remains a global health concern, with 249 million cases and 608,000 deaths being reported by the WHO in 2022. Traditional diagnostic methods often struggle with inconsistent stain quality, lighting variations, and limited resources in endemic regions, making manual detection time-intensive and error-prone. This study introduces an automated system for analyzing Romanowsky-stained thick blood smears, focusing on image quality evaluation, leukocyte detection, and malaria parasite classification.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!